ATRA对A_(549)生物学特性及E-CD/catenins表达的影响  

The Influence of ATRA to the Cell Proliferation and the Expression of E-Cadherin /Ceatenin Complexes of A549Cell

作　　者：张庆玲[1] 王志新[2] 汤珣 

机构地区：[1]第一军医大学基础部实验中心,广州市510515 [2]珠江医院肾内科 [3]新桥医院全军肿瘤中心

出　　处：《中国肿瘤临床》2002年第7期461-464,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金资助(编号:39870350)

摘　　要：目的:探讨诱导分化剂全反维甲酸(ATRA)对肺腺癌A549细胞增殖和E-CD/catenins复合物表达的影响。方法:用细胞生长率分析不同浓度ATRA处理不同时相的A549细胞的增殖特性变化;用免疫组织化学法检测ATRA处理前后A549细胞的E-CD/catenins复合物各成分(E-CD、α-和β-catenin)的表达情况。结果:无ATRA处理组A549细胞增殖力最强,而各处理组细胞增殖能力均有较大降低;免疫组化结果显示无ATRA处理组细胞基本不表达E-CD和α-catenin,而β-catenin主要呈膜表达阳性;处理后的A549细胞E-CD、α-和β-catenin表达水平均有明显增强,并且三者的表达形式均以细胞膜表达为主。结论:ATRA可抑制肺腺癌A549细胞增殖、增强E-CD/catenins复合物表达和膜定位。Objective:To explore the effect of the differentiation-inducing agent all trans-retinoic acid(ATRA)to the cell proliferation and the expression of E-cadherin/catenin complexes in lung adenoma cell A549.Methods:After cultured by different density ATRA in different times,A549cell had been used to analyse the proliferation by the cell proliferative curve,and the expression of E-cadherin,α-catenin andβ-catenin by immunohistochemistry.Results:The proliferation was strongest in non-ATRA treated cells,but it was significantly inhibited in ATRA-treated cells.As compared with untreated A549cells,the expressions of E-cadherin,α-catenin andβ-catenin enhanced significantly in ATRA-treated cells.The expression of E-cadherin andα-catenin changed from negative to strong membranous expression and weak cytoplasmic positive.The allocation ofβ-catenin had not changed as the predominant membranous expression and weak cytoplasmic positive,but the express level enhanced.Conclusion:ATRA could enhance the expression of E-cadherin/catenin complexes and inhibit the multiplication of the lung carcino-ma cell A549.

关 键 词：E-钙粘蛋白 Α-连环素 Β-连环素 肺肿瘤 全反维甲酸 细胞增殖 药理学 膜定位 

分 类 号：R73-36[医药卫生—肿瘤]