胃肠道癌患者外周血和骨髓中微转移检测的意义  

Significance of Cancer Cells in Peripheral Blood and Bone Marrow Detected by Nested RT-PCR in Patients with Gastrointestinal Cancer

作　　者：邬宇飞[1] 夏加增[2] 李辉[1] 尹浩然[3] 林言箴[3] 

机构地区：[1]上海第二医科大学瑞金医院卢湾分院,上海市200020 [2]江苏省无锡市第二人民医院普外科 [3]上海第二医科大学瑞金医院普外科

出　　处：《中国肿瘤临床》2002年第7期489-491,共3页Chinese Journal of Clinical Oncology

摘　　要：目的:探讨胃道肠癌患者外周血和骨髓中微转移的检测及其意义。方法:以CK19作指标,用巢式RT-PCR方法检测75例胃、结肠癌患者术前外周血中癌细胞,同时对其中33例患者骨髓进行检测。结果:75例中,24例(32.0%)外周血中检测出CK19mRNA的表达,其中60例胃癌患者的检出率为30.0%(18/60),15例结肠癌的检出率为40.0%(6/15)。外周血阳性率随着肿瘤分期的增加而增加。其中33例的骨髓阳性率为48.5%,高于这些患者的血液中阳性率(33.3%)。结论:检测外周血和骨髓中微转移可提高胃结肠癌患者临床分期的准确性,帮助判断患者的预后。Objective:To study the detection and significance of micrometastasis of periph-eral blood and bone marrow of patients with gastric or colon carcinoma.Methods:Preoperative peripheral blood of75patients with gastric or colon cancer were searched for CK19mRNA by nested RT-PCR amplification.Among these,33patients were studied simultaneously for mi -crometasta sis in bone marrow.Results:Among75cases,24had positive CK19mRNA expression in periph eral blood(32.0%),and the positive rate was30.0%(18/60)in60gastric caqncer pa-tients and40%(6/15)in13patients with colon cancer.The positive rate was correlated with the stage of tumor,while the positive rate in bone marrow was48.5%in33patients which was high-er than that in the peripheral blood.Conclusion:Detection of micrometastasis in peripheral blood and bone marrow may improve the accuracy of clinical staging of the tumor and help to assess the prognosis.

关 键 词：胃肠道癌 微转移 外周血 骨髓 细胞角蛋白19 预后 临床分期 

分 类 号：R735[医药卫生—肿瘤]