胰岛素作用的跨膜钙离子流变化与人肝癌细胞增殖的关系  

Changes in calcium ion across plasma membrane by insulin related to the proliferation of human hepatocellular carcinoma

作　　者：张海伟[1] 罗英儒[2] 郑佩娥[1] 陈小琳[3] 司徒锐[1] 

机构地区：[1]暨南大学医学院病理学教研室,广东广州510632 [2]暨南大学医学院药理学教研室,广东广州510632 [3]暨南大学医学院病理生理学教研室,广东广州510632

出　　处：《中国病理生理杂志》2002年第7期813-815,共3页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金项目 (No .395 0 0 0 5 6 );广东省"五个一兴教工程"基金资助项目

摘　　要：目的 :揭示胰岛素影响人肝癌细胞增殖的机制。方法 :利用人肝癌细胞系 (HepG - 2 )为靶细胞 ,以原子吸收光谱技术检测胰岛素对靶细胞跨膜钙离子流影响 ,同时结合钙离子拮抗剂 (isoptin)的作用来研究靶细胞的增殖变化。结果 :胰岛素能明显促使靶细胞跨膜钙离子内流而改变其恒态 ,并对靶细胞增殖有明显的促进作用 ;而钙离子拮抗剂却明显抑制其细胞的增殖。结论 :胰岛素促使人肝癌细胞增殖的机制与其细胞跨膜钙离子流恒态的变化密切相关。AIM: To examine the effect of insulin on the proliferation of human hepatocellular carcinoma cell and its possible mechanisms. METHODS: The human hepatocellular carcinoma cell line(HepG-2) was used to study the changes in calcium ion across plasma membrane (Ca 2+ APM)under the action of insulin by the assay of atomic absorption spectrum, and in the proliferation under the action of insulin and calcium ion antagonist (isoptin). RESULTS: The influx of Ca 2+ APM and the proliferation was increased after insulin administration, but the proliferation was inhibited by isoptin. CONCLUSION: Changes in the homeostasis of calcium ion across plasma membrane was involved in the effect of insulin on the proliferation of human hepatocellular carcinoma.

关 键 词：跨膜钙离子流 细胞增殖 肝细胞瘤 胰岛素 肝癌 

分 类 号：R735.7[医药卫生—肿瘤]