建立超敏ELISA-双酶底物循环法检测tau蛋白  

Development of an Ultrasensitive ELISA-dienzyme Substrate Recycle Assay for Measurement of Cerebrospinal Fluid Tau

作　　者：胡元元[1] 周新文[1] 何善述[1] 王建枝[1] 

机构地区：[1]华中科技大学同济医学院,武汉430030

出　　处：《生物化学与生物物理进展》2002年第4期635-639,共5页Progress In Biochemistry and Biophysics

基　　金：国家杰出青年科学基金 (3 992 5 0 12 ) ;国家基础研究发展规基金 (G19990 5 40 0 7) ;国家教育部教技司基金 (2 0 0 1 171号 )资助项目~~

摘　　要：利用酶联免疫吸附法 (ELISA)的高特异性和双酶底物循环的高灵敏度 ,建立了ELISA 双酶底物循环扩增检测法 .用传统ELISA测定纯化tau蛋白和阿尔茨海默病异常磷酸化tau蛋白的范围值分别为 1～ 32ng和 0 2～ 10ng ,而此法的测定范围值分别为 0 75～ 2 0 0pg和 0 5～ 5 0 pg ,比传统ELISA的灵敏度分别提高 130 0倍和4 0 0倍 ,可测定范围值亦分别扩大了 8 5倍和 2倍 .可准确测定阿尔茨海默病患者脑脊液样品中的微量tau蛋白和异常磷酸化tau蛋白 ,为阿尔茨海默病的早期诊断和鉴别诊断提供了新技术 .On the basis of conventional enzyme linked immunosorbent assay (ELISA) and dienzyme substrate recycle, An highly specific and ultrasensitive biochemical assay, named ELISA dienzyme substrate recycle was developed. The detective range of conventional ELISA for measuring recombinant tau and abnormally phosphorylated tau purified from Alzheimer disease brain was 1ng to 32 ng. It was 0 75pg to 200pg and 0 5pg to 50pg by ELISA dienzyme substrate recycle. Compared with the conventional method, the sensitivity of the technique established in the present study increased 400 and 1300 times, with a enlarged detective range of 8 5 and 10 times, respectively. It could detect abnormally phosphorylated tau in cerebrospinal fluid of Alzheimer disease and used effectively for the diagnosis of the disease.

关 键 词：超敏ELISA-双酶底物循环法 检测 TAU蛋白 阿尔茨海默病 

分 类 号：R749.16[医药卫生—神经病学与精神病学]