改造中国仓鼠卵巢细胞  

Modification of Chinese Hamster Ovary Cells

作　　者：来大志[1] 齐连权[1] 于长明[1] 王海涛[1] 陈薇[1] 

机构地区：[1]北京微生物流行病研究所,北京100071

出　　处：《生物工程学报》2002年第4期415-419,共5页Chinese Journal of Biotechnology

摘　　要：与原核细胞、酵母细胞以及昆虫细胞相比 ,中国仓鼠卵巢细胞 (CHO)作为宿主细胞表达的外源蛋白最接近其天然构象 ,因而CHO细胞表达系统是生物工程制药最为理想的表达系统。但这种系统也存在诸多缺点。如在大规模培养中CHO细胞会面临着对无血清培养基的适应性差、细胞无限度增殖以及细胞凋亡等很多难题。所以除了在培养基、培养条件和表达载体方面下功夫优化该系统外 ,对CHO细胞本身进行改造已成为优化CHO表达系统的另一热点。Chinese hamster ovary cells (CHO) are preferable to prokaryotic, yeast or insect cells as hosts for biopharmaceutical production due to the products are more similar to their natural conformation. However, CHO cells confront tremendous difficulties when cultured in large scale such as mal-adaptation to serum-free medium, apoptosis and over-growth without limitation. So in addition to optimizing CHO system in respect of medium, environment and expression vector, modification of CHO cells themselves has drawn more and more attention. Here the main progress in CHO-modification is reviewed.

关 键 词：改造 中国仓鼠卵巢细胞 CHO细胞 大规模培养 细胞凋亡 细胞黏附 细胞周期 

分 类 号：Q813[生物学—生物工程]