云南五个少数民族的CSF1PO、TPOX和TH01基因座遗传多态性研究  

Distribution of CSF1PO,TPOX and TH01 loci in five minority populations in Yunnan of China

作　　者：邹苹 邹浪萍 申滨 杨燕[3] 李德林[3] 褚嘉祐[4] 

机构地区：[1]昆明市妇幼保健医院,650031 [2]云南省高级人民法院司法鉴定科学技术研究所 [3]昆明医学院 [4]中国医学科学院医学生物学研究所

出　　处：《中华医学遗传学杂志》2002年第4期332-335,共4页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金 (39760 0 35;3999342 0 );美国中华医学基金 CMB项目 (98- 675)~~

摘　　要：目的　了解云南阿昌族、德昂族、布朗族、普米族和基诺族等 5个少数民族的 CSF1PO、TPOX和 TH0 1基因座遗传多态性分布。方法　应用复合扩增技术对 CSF1PO、TPOX和 TH0 1等 3个短串联重复序列 (short tandem repeat,STR)基因座进行扩增 ,采用高分辨率的聚丙烯酰胺凝胶电泳分离、银染法显影技术 ,对云南上述 5个少数民族的基因座进行遗传多态性调查。结果　获得了云南省特有的阿昌族、德昂族、布朗族、普米族和基诺族等 5个少数民族的 CSF1PO、TPOX和 TH0 13个基因座的基因频率分布资料。结论　 CSF1PO、TPOX和 TH0 13个基因座基因频率分布与 Hardy- Weinberg平衡吻合良好 。Objective To investigate the distribution of CSF1PO, TPOX and TH01 in 5 minority populations only resided in Yunnan province. Methods DNA extraction from bloods and multiplex amplification of CSF1PO, TPOX and TH01 were carried out. The technique of denaturing polyacrylamide gel electrophoresis and silver staining method were used. Results The data on the distribution of allele frequencies of 3 loci(CSF1PO, TPOX and TH01) in Achang,Deang,Bulang,Pumi and Jino were collected and analyzed. Conclusion The allele distribution of the loci were in good agreement with the Hardy Weinberg equibrium. A satisfactory result was obtained and some significant genetics differences were noticed in different populations.

关 键 词：CSF1PO TPOX TH01 中国少数民族 基因座 遗传多态性 复合扩增技术 

分 类 号：R394[医药卫生—医学遗传学]