三个群体MICA基因外显子2、3和4的多态性研究  

Analysis on polymorphism in exons 2,3 and 4 of the MICA gene in three different Chinese populations

作　　者：龚卫娟[1] 范丽安[1] 杨珏琴[1] 许玲娣[1] 姚芳娟[1] 

机构地区：[1]上海第二医科大学上海市免疫学研究所,200025

出　　处：《中华医学遗传学杂志》2002年第4期336-339,共4页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金 (39970 742 );上海市教委资助项目(99QB68);联合 -利华资助项目 (991 1 )~~

摘　　要：目的　调查上海地区汉族、云南傣族和新疆维吾尔族 3个群体 MICA基因外显子 2、3和 4的多态性。方法　采用聚合酶链反应 -序列特异的寡核苷酸探针杂交 (polymerase chain reaction andsequence- specific oligonucleotide probing,PCR- SSOP)方法 ,分析 183名汉族、4 1名傣族和 6 6名维吾尔族正常人群的 MICA胞外区等位基因多态性。结果　分别在汉族、傣族和维吾尔族中检测出 10、7和 9个MICA等位基因 ,其中 MICA* 0 0 8在汉族和维吾尔族中频率最高 ,而傣族中 MICA* 0 10的频率最高。 3个民族 MICA等位基因分布方式各不相同 ,而且维吾尔族的等位基因分布与另外两个民族相比 ,差异具有显著性。结论　MICA等位基因分布方式具有民族地区特异性。Objective: To detect genetic polymorphism in the exons 2 to 4 of the MICA gene in Chinese Han population in Shanghai, Dai population in Yunnan province and Uygur population in Xinjiang province respectively. Methods: DNA samples from 183 random healthy individuals in Han population, 41 in Dai population and 66 in Uygur population were genotyped by using the polymerase chain reaction-sequence specific oligonucleotide probing (PCR-SSOP) method. Results: In total, 10,7 and 9 alleles of MICA were observed in Han, Dai and Uygur population respectively. MICA*008 was the most common allele in the population of both Han and Uygur, whereas MICA*010 was the most popular one in Dai population. Han and Dai had a bit similar distribution pattern (χ2=12.809, P=0.046), in contrast with Han to Uygur (χ2=58.499, P=0) and Dai to Uygur (chi;2=49.273, P=0). Conclusion: MICA gene displayed different allele distributions in different populations.

关 键 词：MICA基因 多态性 人类白细胞抗原B 连锁不平衡 PCR-SSOP 

分 类 号：R394[医药卫生—医学遗传学]