白细胞与早期糖尿病视网膜病变关系的研究  

Study on Relationship between Leukocytes and Early Diabetic Retinopathy

作　　者：白宁艳[1] 唐仕波[1] 林健贤[1] 罗燕[1] 林少芬[1] 朱邦豪[2] 

机构地区：[1]中山大学中山眼科中心,广州510060 [2]中山大学中山医学院药理教研室,广州510089

出　　处：《眼科学报》2002年第2期71-75,共5页Eye Science

基　　金：本研究受"广东省眼科视觉科学重点实验室"专项基金资助(B60105)

摘　　要：目的:探讨白细胞在早期糖尿病大鼠视网膜微血管中粘附、堆积,以致引起微循环障碍的作用。方法:健康成年雄性Wistar大鼠16只,随机分成正常对照组和链尿佐菌素（Streptozotocin,STZ）糖尿病3个月组各8只;对其视网膜消化铺片进行形态学观察及白细胞共有抗原（CD45）单克隆抗体免疫组织化学研究。结果:发现3个月病程的STZ诱导的糖尿病大鼠视网膜毛细血管已出现毛细血管管径不规则,呈节段性膨大或囊样扩张等形态学改变;视网膜毛细血管中CD45的表达明显增加,白细胞呈串珠样粘附和堆积。结论:3个月病程的STZ糖尿病大鼠可出现视网膜微血管改变;白细胞在糖尿病视网膜病变的发生发展中起着重要作用。Purpose: To explore the leukocytic functions in adhesion, stacking and causingmicrocirculatory disorder in retinal microvessels in early diabetic rats.Methods: Sixteen healthy adult male Wistar rats were randomly divided into normalcontrol group and 3 months group of diabetes (STZ) . We carried out morphologicalobservations and CD45(Leukocyte Common Antigen) monoclonal antibody immunohist-ochemical studies of the retinal digest preparations.Results: Morphological changes including capillary varix, irregularity of capillarycaliber, was found in the retinal blood capillaries in diabetic rats at 3 months. Furthermore, the expression of CD45 was significantly increased in the diabetic retinalmicrovessels, which were found leukocytes adhering and stacking.Conclusion: Changes of retinal microvessels can be found in STZ diabetic rats at 3months. The leukocytes were important in the onset and development of diabeticretinopathy(DR).

关 键 词：糖尿病视网膜病 白细胞 大鼠 链尿佐菌素 

分 类 号：R774.1[医药卫生—眼科] R587.2[医药卫生—临床医学]