多参数诊断复发急性髓性白血病的再生耐药  

Diagnosis of Regrowth Drug Resistance by Multiple Parameters in Relapsed Acute Myeloid Leukemia

作　　者：何明生[1] 陈燕[1] 喻东姣[1] 李慧玉[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院血液病研究所,武汉430022

出　　处：《华中科技大学学报（医学版）》2002年第4期409-411,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：卫生部科学研究基金资助项目 (No. 1996 - 2 - 10 8)

摘　　要：为研究临床多参数诊断急性髓性白血病 (AML)再生耐药的标准和方法 ,选择 2 0例复发 AML,用流式细胞仪 (FCM)检测细胞周期中 S期细胞百分比 (S% )和细胞内柔红霉素 (DNR)浓度 ,检测白血病细胞集落形成单位(CFU- L)和 MTT药敏试验 ,用碱性磷酸酶抗碱性磷酸酶法 (APAAP)检测 P- 170蛋白的表达 ,经多参数诊断 2 0例复发 AML,11例为再生耐药。提示 :多参数检测可作为诊断 AML 再生耐药的方法 ,并提出了诊断标准 ,为 AMLTo investigate clinically diagnostic criteria and methods of regrowth drug resistance (RDR) in acute myeloid leukemia (AML), 20 patients with relapsed AML were studied with multiple parameter methods,namely to determine the percentage of S phase cell(S %)in cell cycle, the intracellular concentration of daunorubicin (DNR) by flow cytometry(FCM),leukemic cell colony forming unit(CFU L), MTT drug sensitivity test and expression of P 170 proteins by alkaline phosphatase antialkaline phosphatase (APAAP) assay. Eleven out of 20 patients were diagnosed as having RDR. These studies suggested that the diagnosis with multiple parameters may be a way to detect RDR in AML.The diagnostic criteria were proposed.

关 键 词：再生耐药 急性髓细胞性白血病 诊断标准 

分 类 号：R733.710.4[医药卫生—肿瘤]