非同位素法显示DNA合成期细胞的研究  

A comparative study of isotopic and nonisotopic methods for cell kinetcs study

作　　者：陈卫三 段朝军[1] 胡有秋[1] 陈主初[1] 

机构地区：[1]湖南医科大学肿瘤研究所

出　　处：《中国病理生理杂志》1991年第6期646-649,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金

摘　　要：本文用~3H-TdR和BudR同时掺入体外培养细胞并先后以免疫化学和放射自显影方法显示DNA合成期标记的细胞。结果表明,BudR免疫化学法显示的S期标记细胞与~3H-TdR放射自显影法标记的结果高度吻合,进一步证明具有独特的优点,可以取代经典的同位素法进行细胞动力学研究。同时,本文还对贴壁细胞和悬浮培养细胞的BudR掺入条件作了初步探讨。Hela cells and human peripheral lymphocells were labeled with tritiated thymidine (~3H-TdR) and 5-bromodeoxyuridine (BudR) simultaneously for 1-12 hours. The S phase (DNA synthesis) cells were then detected by monoclonal antibody against BudR (BU-1) through immunocytochemistry and autoradiography, respectively. The labeling indexes of BUdR and ~3H-TdR in the same sample were almost the same. This nonisotopic technique offers a faster and relatively simplar alternative method for determing labeling indexes of human tumors.

关 键 词：溴脱氧脲苷 DNA 细胞周期 肿瘤 

分 类 号：R73－35[医药卫生—肿瘤]