单眼视觉剥夺幼猫外侧膝状体中一氧化氮合酶变化的组织化学研究  

Histochemical studies on the change of nitric oxide synthetase in lateral geniculate nucleus of monocular deprived kittens

作　　者：高建华[1] 张东杲[2] 宫枢政[1] 

机构地区：[1]解放军成都军区昆明总医院眼科,650032 [2]第四军医大学唐都医院眼科

出　　处：《中华眼科杂志》2002年第8期476-479,W001,共5页Chinese Journal of Ophthalmology

摘　　要：目的　观察一氧化氮合酶 (nitricoxidesynthase ,NOS)在正常和单眼视觉剥夺幼猫外侧膝状体中的变化 ,探讨一氧化氮 (nitricoxide ,NO)在弱视形成过程中的作用。方法　用尼克酰胺腺嘌呤二核苷酸黄递酶 (nicotinamidadeninedinucleotidephosphate diaphorase,NADPH diaphorase)组织化学染色法观察正常组和单眼视觉剥夺组幼猫外侧膝状体中NOS的变化。结果　 (1)正常组幼猫外侧膝状体各层中无NOS阳性细胞 ,但可见NOS阳性纤维 (轴突和末梢 ) ;(2 )单眼视觉剥夺组幼猫外侧膝状体各层中可见NOS阳性纤维 (轴突和末梢 )。两组幼猫外侧膝状体各层NOS阳性纤维 (轴突和末梢 )NADPH diaphorase染色无明显差别。 (3)单眼视觉剥夺组幼猫外侧膝状体的非剥夺层中可见条状分布的NOS阳性细胞 ,平均密度为 (16 80± 2 6 4 )个 /mm2 ,平均面积为 (44 5± 96 ) μm2 /个 ,无长树突 ,胞浆染色重而细胞核无染色 ;剥夺层偶见NOS阳性细胞 ,平均密度为 (1 80± 1 4 7)个 /mm2 ,平均面积为 (30 7± 75 )μm2 /个。Objective To observe the change of nitric oxide synthetase (NOS) in lateral geniculate nucleus of normal and monocular deprived kittens and to discuss the role of nitric oxide in the etiopathology of amblyopia. Methods The distribution of nitric oxide synthetase in lateral geniculate nucleus (LGN) of normal and monocular deprived kittens had been studied by using nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase histochemical method (NDP). Results In normal kittens, NOS positive cells were not seen in any lamina of LGN, but NOS positive fibers were discovered. In monocular deprived kittens, NOS positive fibers were seen in every layers of LGN, in the nondeprived laminae of LGN there was strip shaped distribution of NOS positive cells, and in the deprived laminae the NOS positive cells were occasionally seen. Conclusion NOS might act as a new class of neurotransmitter and be involved in the formation of emblyopia.

关 键 词：单眼视觉剥夺 幼猫 外侧膝状体 一氧化氮合酶 弱视 组织化学染色法 

分 类 号：R770.2[医药卫生—眼科]