葡萄籽原花青素对鼠伤寒沙门氏菌的抗诱变作用  

ANTIMUTAGENIC EFFECT OF PROCYANIDINS FROM VITIS VINIFERA SEED IN SALMONELLA TEST

作　　者：孙志广[1] 赵万洲[2] 陆茵[2] 唐玲芳[3] 张珍玲[3] 张世玮[2] 

机构地区：[1]江苏省中医院消化内科,江苏南京210029 [2]南京中医药大学中药学院 [3]南京医科大学应用毒理教研室

出　　处：《癌变．畸变．突变》2002年第3期191-194,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：中国博士后科学基金资助项目 (No .1997)

摘　　要：目的 :观察葡萄 (Vitisvinifera)籽原花青素的抗诱变作用。方法 :以SalmonellatyphimuriumTA97、TA98、TA10 0 、TA10 2作为标准试验菌株进行Ames试验。结果 :在不加S9条件下 ,原花青素能显著抑制Dexon诱发的TA97、TA98回复突变 ,叠氮钠诱发的TA10 0 回复突变 ,丝裂霉素C诱发的TA10 2 回复突变 ;在不加S9条件下 ,原花青素能显著抑制 2 氨基芴诱发的TA97、TA98及TA10 0 回复突变 ,而对 1,8 二羟基蒽醌诱发的TA10 2 回复突变抑制作用较强。结论Purpose: Antimutagenic effect of procyanidins from Vitis vinifera seed was studied. Methods: A series of histidine requiring strains TA\-\{97\}, TA\-\{98\}, TA\-\{100\} and TA\-\{102\} were used for antimutagenicity test. Several mutagenic agents such as Sodium Azide(NaN\-3), Mitomycine C(MMC), Dexon, 2 AF, 1,8 Dihydroxyanthrachinon were used to make mutagenic model and antimutagenic effects of procyanidins were observed. Every tester strain was divided into model group, spontaneous mutation group and drug groups. Results: The results showed that procyanidins inhibited mutagenic effects induced by the most of the model agents, but little inhibitory effect was observed on TA\-\{102\} reverse mutation induced by 1,8 Dihydroxyanthrachinon(+S\-9). Conclusion: Antimutagenesis is one of mechanism chemoprevention of cancer of procyanidins.

关 键 词：原花青素 葡萄 AMES试验 诱变剂 伤寒 沙门氏菌 抗诱变作用 

分 类 号：R259.163[医药卫生—中西医结合] R285[医药卫生—中医内科学]