热变性温度法测定蚤类DNA中G+C含量的初步研究  

Preliminary studies on the determination of the G+C mol% of flea DNA from its thermal denaturation temperature

作　　者：王善青[1] 尤洪涛[2] 吴厚永[2] 郝桂英[2] 林万明[2] 郭兆彪[2] 

机构地区：[1]山西医学院 [2]军事医学科学院微生物流行病研究所

出　　处：《中国地方病学杂志》1991年第6期325-327,共3页Chinese Jouranl of Endemiology

摘　　要：本文采用改进的氯仿处理法提取两种蚤类 DNA,简便、快速、经济、得到 OD_(260)/OD_(280)的比值均大于1.9,琼脂糖凝胶电泳后基部呈现单带的纯 DNA;两种蚤 DNA 在1×SSC 溶液中的热变性温度(Tm)和 G+Cmol%分别如下:印鼠客蚤雌虫:82.2℃,31.5%;雄虫:82.3℃,31.7%;方形黄鼠蚤松江亚种雌虫:83.6℃,34.9%;雄虫83.6℃,34.9%。This paper reports our work on the use of the thermal denaturation temperatu-re(Tm)method in determining G+C mol% of flea DNA.Comparatively pureDNA of Xenopylla cheopis Citellophylus tesquerum sungris was obtained byimproved marmur method.The purity of DNA preperation was proved by electro-phoresis and value of UV spectrometry.The Tm and G+C mol% of the two species of fleas were as follows(the dilue-nt liquid is 1×SSC):Xenopsylla cheopis female,82.2℃,31.5%;male,82.3℃31.7%;Citellophylus tesquerum sungris female,83.6℃,34.9%;male,83.6℃, 34.9&。

关 键 词：蚤类 热变性温度 DNA G+C含量 

分 类 号：R384.4[医药卫生—医学寄生虫学]