支气管动脉灌注治疗对中央型肺癌微血管密度及血管内皮生长因子的影响  

The effect of bronchial artery infusion therapy on microvascular density and vascular endothelial growth factor expression in central bronchogenic carcinoma

作　　者：陈雁[1] 刘玉清[2] 周纯武[1] 李槐[1] 张洵[3] 刘秀云[3] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤医院放射诊断科,北京100021 [2]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院放射科 [3]中国医学科学院中国协和医科大学肿瘤医院病理科,北京100021

出　　处：《中华放射学杂志》2002年第8期716-719,共4页Chinese Journal of Radiology

基　　金：国家"九五"攻关项目 (96 90 7 0 3 0 3 )

摘　　要：目的　观察支气管动脉灌注 (BAI)治疗对中央型支气管肺癌肿瘤血管生成的影响。方法　 2 2例中央型支气管肺癌 ,治疗前均经支气管镜检查证实 ,经BAI治疗后行手术切除 ,用免疫组织化学 (以下简称免疫组化 )方法分别对经支气管镜及BAI后手术切除获得的标本进行染色 ,观察肿瘤微血管密度 (MVD)及血管内皮生长因子 (VEGF)的表达 ,分别于BAI治疗前后近期行CT扫描观察肿瘤最大径及临床分期 ,并进行统计学分析。结果　BAI治疗前、后肿瘤组织MVD分别为 16 84±7 6 8、2 1 85± 6 16 ,二者差异无显著性意义 (P >0 0 5 )。BAI治疗后肿瘤组织VEGF的表达明显下降(P <0 0 1)。BAI治疗前、后病灶最大径分别为 (4 84± 1 73)cm、(3 78± 1 5 5 )cm ,二者差异具有极显著性意义 (P <0 0 1)。BAI疗前、后肿瘤临床分期的差异无显著性意义 (P >0 0 5 )。BAI治疗前肿瘤VEGF的表达与肿瘤的大小呈负相关 (r =- 0 340 ,P <0 0 5 ) ;BAI治疗前后肿瘤大小的变化与肿瘤MVD的变化呈正相关 (r =0 316 ,P <0 0 5 ) ;BAI治疗后肿瘤VEGF的表达与肿瘤的大小无相关性(r=- 0 16 8,P >0 0 5 ) ;BAI治疗前、后肿瘤MVD、VEGF与临床分期无相关性 (r=0 0 0 9～ 0 2 6 9,P >0 0 5 )。Objective To observe the effect of BAI therapy on tumor angiogenesis of central bronchogenic carcinoma Methods Twenty two patients with central bronchogenic carcinoma undergone radical resection after bronchial artery infusion (BAI) therapy The diagnosis in all patients was histologically confirmed by the examination of fiberoptic bronchoscopy(FOB) before therapy All samples obtained from FOB biopsy and surgical excision were stained by immunohistochemical method Microvascular density (MVD) and vascular endothelial growth factor (VEGF) protein expression in tumor tissue were measured respectively CT scanning was performed before and after BAI therapy respectively in all patients to observe the maximum diameter of tumor and clinical staging The statistical analysis was performed by the software of SPSS for windows 8 0 Results MVD of tumor tissue before and after BAI therapy was 16 84±7 68 and 21 85±6 16, respectively, and the results were not statistically significant The VEGF expression, however, was significantly decreased after BAI therapy ( P <0 01). The maximum diameter before and after BAI therapy was (4 84±1 73) cm and (3 78±1 55) cm respectively, the results were statistically significant ( P <0 01) The clinical staging before and after BAI therapy was not statistically significant ( P >0 05) The VEGF expression before and after BAI therapy showed negative correlation with the maximum diameter of tumor( r =-0 340, P <0 05) The change of the maximum diameter of tumor showed positive correlation with the change of MVD( r =0 316, P <0 05). There was no correlation between the VEGF expression and the maximum diameter of the tumor after BAI therapy( r =-0 168, P >0 05). MVD and VEGF expression showed no correlation with the clinical staging before and after BAI therapy ( r = 0 009- 0 269, P >0 05) Conclusion BAI therapy may inhibit the tumor angiogenesis of central bronchogenic carcinoma

关 键 词：支气管动脉灌注 治疗 中央型肺癌 内皮生长因子 免疫组织化学 病理学 微血管密度 

分 类 号：R734.2[医药卫生—肿瘤]