不同组织学类型人肺癌细胞系的维甲酸受体表达  

Comparative study on the expression of retinoic acid receptors (RARs) in the four cell lines of human pulmonary carcinoma

作　　者：蔡小强[1] 谢作煊[1] 

机构地区：[1]暨南大学医学院病理学教研室,广州510632

出　　处：《临床与实验病理学杂志》2002年第3期316-318,共3页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的 :观察来源于不同组织学类型的人肺癌细胞系 (肺巨细胞癌细胞系PLA 80 1,肺鳞状上皮细胞癌细胞系C 5 7,个旧肺腺癌细胞系GLC 82和肺腺癌细胞系PC 840 45 )的维甲酸受体的含量和分布特征。方法 :用ELISA和免疫组化显色计算机图像分析方法 ,对 4株具有不同侵袭潜能、不同组织学类型的肺癌细胞系的维甲酸受体 (RARs、RARβ和RXRs)进行检测。 结果 :4株细胞系的RARβ表达极少 ,甚至缺如 ;RARs主要分布在癌细胞质中 ,但RXRs主要位于细胞核内 ,表达水平较高 ;RARs和RXRs在PLA 80 1细胞中表达最低。结论 :肺癌中普遍存在着RARβ的缺失与RARs的表达分布异常 ,这些改变可能与肺癌的异常分化有关 ,也可能是肺癌细胞对维甲酸诱导分化不敏感的重要原因。RXRs较高水平的表达主要位于核内 ,提示用 9Purpose To observe the expression and distributive characteristics of RARs in the four cell lines of human pulmonary carcinoma including pulmonary large cell carcinoma cell line PLA-801, pulmonary squamous cell carcinoma cell line C-57,Gejiu lung adenocarcinoma cell line Glc-82 and pulmonary adenocarcinoma cell line PC-84045,which originated from different histologic type. Methods The analyses of RARs,RARβ and RXRs were conducted by ELISA and immunohistochemistry technique combining computer image analysis. Results (1) The level of RARβ in all four cell lines was very low, even lost. (2)RARS was mainly located in the cell plasma of the four cell lines, but RXRs mainly in the cell nucleus and the level of its expression relatively higher. (3)The level of RARs and RXRS in the PLA-801 cell line was lowermost. Conclusions The loss of RARβ and the abnormal location of RARs exist commonly in the lung carcinoma cell, and may be closely related to abnormal differentiation of pulmonary carcinoma, and prbably was a important cause uninfluenced to all-tran-retinoid acid(AT-RT), and the effect of inducing differentiation with 9-cis-retinoic acid might be better than that with AT-RT.

关 键 词：组织学类型 肺癌 细胞系 维甲酸受体 肺肿瘤 

分 类 号：R734.2[医药卫生—肿瘤]