生物素标记的6种cDNA探针检测H22、EAC及其克隆细胞株mRNA的表达  

The difference of mRNA expression In H22 and EAC and their cloned cells

作　　者：吕占军[1] 李秀霞 刘秋燕[1] 王秀芳[1] 

机构地区：[1]河北医科大学实验动物学部,石家庄050017 [2]河北省免疫学会

出　　处：《肿瘤防治研究》2002年第4期261-263,共3页Cancer Research on Prevention and Treatment

基　　金：国家"九五"攻关课题 (96 A2 3 0 6 0 3)

摘　　要：目的　检测H2 2、EAC瘤细胞及其克隆细胞株mRNA的表达。方法　用生物素标记的 6种cDNA探针 ,细胞玻片原位杂交的方法。结果　H2 2与生物素标记的 4种探针杂交阳性 ,其克隆细胞株H2D8、H2G4分别与 3及 1种探针杂交阳性 ;EAC细胞株与 4种探针杂交阳性 ,克隆细胞株E2G8与 2种探针杂交阳性 ,E2C6克隆细胞株与 6种探针杂交均阴性。Objective To examine the difference of mRNA expression in H22 and EAC strains and their cloned cells. Methods The probes labeled by biotin were used in situ hybridization to detect the expression of 6 kinds of mRNA. Results 4 kinds of mRNAs were found to be exist in H22 cells,3 and 1 kinds of mRNAs in H2D8 and H2G4 cloned cells, respectively; 4 kinds of mRNAs were found to be exist in EAC cells, 2 kinds of mRNAs in E2G8 cloned cell; No mRNA in E2C6 cloned cell. Conclusion The expression of mRNAs was different in H22,EAC and their cloned cell strains.

关 键 词：瘤细胞株 克隆细胞 原位杂交 分子探针 CDNA探针 mRNA 生物素 

分 类 号：R730.21[医药卫生—肿瘤]