苏云金芽孢杆菌的分离及快速鉴定  

Isolation and Quick Identification on Bacillus thuringiensis

作　　者：李达娜[1] 钱龙[1] 

机构地区：[1]贵州大学生物技术学院生物科学与技术系,贵阳550025

出　　处：《贵州农业科学》2002年第4期34-35,共2页Guizhou Agricultural Sciences

基　　金：贵州大学校基金 [贵大 2 0 0 1 (0 91 ) ]

摘　　要：从土壤样品中分离出 5 7株疑似苏云金芽孢杆菌的菌株 ,采用地高辛标记 Cry IAb基因 5′端的72 6 bp Eco R I片段为探针 ,进行菌株质粒 DNA斑点杂交 ,筛选出 2 5株阳性菌株。其生理生化特征与伯杰氏细菌鉴定手册第八版的描述相符 ,确认为苏云金芽孢杆菌菌株。strains suspected Bacillus thuringiensis were isolated from soil. The strains quality grain DNA was spot hybridized with the 726bp EcoR I part of CryIAb gene 5′ as the probe and mark from DIG DNA Labeling. The 25 Bacillus thuringiensis strains and DNA probe were positive and were identified according to the Bergy's manual.

关 键 词：苏云金芽孢杆菌 分离 快速鉴定 

分 类 号：S476.11[农业科学—农业昆虫与害虫防治] Q939.124[农业科学—植物保护]