海马MF-CA3突触习得性LTP诱导后PPF效应的变化  

Change of Paired-Pulse Facilitation after Behavioral LTP at MF-CA3 Synapses in Hippocampus

作　　者：严文文[1] 刘文晓[1] 黄俊柅[1] 肖鹏[1] 

机构地区：[1]华南师范大学生命科学学院,广州510631

出　　处：《华南师范大学学报（自然科学版）》2014年第4期82-86,共5页Journal of South China Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(39300039)

摘　　要：采用电生理学与行为学结合的方法,通过慢性微电极埋植技术及双脉冲检测技术,观察大鼠海马MF-CA3(Mossy fibers-CA3)突触在明暗辨别学习过程中形成习得性长时程增强(Long-term potentiation,LTP)后双脉冲易化(Paired-pulse facilitation,PPF)效应的变化.结果表明:MF-CA3突触习得性LTP形成前的PPF易化率为138.36%±9.25%,形成后则为114.75%±8.42%,差异有统计学意义(P<0.01),而基线对照组在长达7天的检测中,PPF易化率稳定在140%左右.研究结果说明MF-CA3突触的习得性LTP的表达可能与突触前递质释放的改变有关.The technique of paired-pulse test combined with electrophysiology and behavioral learning was used to examine the effect of behavioral long-term potentiation （LTP） on paired-pulse facilitation （PPF） efficacy at hippocampal MF-CA3 synapses after behavioral learning. The results showed that in baseline （non-training） group, the PPF ratio was stable at the level of 140% or so. However, in training group, the PPF ratio was 138.36%±9. 25% before learning-dependent LTP and was significantly decreased to 114.75%± 8.42% after learning-dependent LTP induction （P 〈0. 01 ）. These results suggest that the expression of behavioral LTP at MF-CA3 synapses may be related to presynaptic neurotransmitter release.

关 键 词：习得性LTP PPF MF-CA3 海马 大鼠 

分 类 号：Q42[生物学—神经生物学]