产孢海洋真菌等离子体诱变活性突变株筛选改进  

Screening Plasma-Induced Bioactive Mutants of Sporogenous Marine Fungus Using a Modified Method

作　　者：温利雪 张翼[1,2] 穆军[1] 冯妍[1] 韩金媛 修志龙[2] 

机构地区：[1]大连交通大学环境科学与工程学院,辽宁大连116028 [2]大连理工大学生命科学与技术学院,辽宁大连116024

出　　处：《大连交通大学学报》2014年第4期87-92,共6页Journal of Dalian Jiaotong University

基　　金：国家自然科学基金资助项目(20902009);中国博士后科学基金资助项目(2011M500051;2012T50243);辽宁省教育厅优秀人才支持计划资助项目(2009R08)

摘　　要：以具有抗肿瘤抗菌活性的产孢子海洋真菌Aspergillus unguis DLEP2008001为出发菌株,对其采用介质阻挡放电等离子体技术进行了诱变处理.在采用肿瘤细胞替代模型——DNA损伤修复基因缺陷型的大肠杆菌E.coli AB3027进行突变株活性筛选的过程中,比较了三种筛选方案,最终确定了一种使用96孔板液体摇床发酵的筛选方法,该方法与通常的液体深层发酵有较好的对应性,且方便易行.通过该方法,灵敏快捷的筛选出了基于DNA损伤机制的高活性突变株,同时也显示介质阻挡放电等离子体技术对提高该菌株生物活性具有显著效果.A marine sporogenous fungal strain Aspergillus unguis DLEP2008001 with anticancer and antibacterial activity was taken as a starting strain to be mutagenized by dielectric barrier discharge plasma technology （DBD）.A DNA damage repairing gene deficient E.coli strain AB3027 was used to screen the mutants of DLEP2008001.During the screening,three different screening methods were compared,and a method using shaking liquid fermentation in 96-well microtiter plates was determined to be the final solution.This method has good correlation with ordinary liquid submerged fermentation and convenience to be carried out.Using this screening method,several mutants with high activity were selected,based on DNA damage mechanism.Meanwhile,the results also exhibits the DBD plasma remarkable effect in improving this strain bioactivity.

关 键 词：等离子体 诱变育种 活性筛选 微孔板摇床发酵 滤纸片法 

分 类 号：TQ920[轻工技术与工程—发酵工程] Q936[生物学—微生物学]