SREBP-1C、GRP-94在非酒精性脂肪肝中的表达及意义  

Expressions of SREBP-1C and GRP-94 in human nonalcoholic fatty liver

作　　者：俞泽元[1] 赵荣荣[2] 盖大伟[1] 罗长江[1] 焦作义[1] 

机构地区：[1]兰州大学第二医院普外一科,甘肃兰州730000 [2]兰州大学第一医院感染科,甘肃兰州730000

出　　处：《中国现代医学杂志》2014年第17期23-27,共5页China Journal of Modern Medicine

摘　　要：目的探讨非酒精性脂肪肝中肝细胞固醇调节元件结合蛋白(SREBP-1C)与葡萄糖调节蛋白94(GRP-94)的表达变化,评价内质网应激蛋白对肝细胞脂质代谢的影响。方法应用半定量逆转录聚合酶链反应(RT-PCR)法和免疫组织化学法检测18例非酒精性脂肪肝患者和10例正常对照肝组织中SREBP-1C与GRP-94基因和蛋白表达,肝活检评分其炎症和纤维化。结果与正常对照组比较,非酒精性脂肪肝患者肝组织SREBP-1c与GRP-94基因和蛋白表达明显增强(P<0.01)。结论非酒精脂肪肝引起的肝脏损害可能与SREBP-1c和GRP-94表达有关,它们参与脂肪肝的发生,这可能是该病的发病机制之一。【Objective】 To explore the expressions of sterol regulatory element binding protein-1C（SREBP-1C） and glucose-regulated protein 94（GRP-94） in human nonalcoholic fatty liver（NAFLD） and to evaluate the effects of endoplasmic reticulum stress proteins on hepatocytes lipids metabolism. 【Methods】 The expressions of SREBP-1C and GRP-94 in hepatic tissues were assessed in 24 patients with NAFLD and 8controls without liver disease using the RT-PCR and immunohistomistry. Liver biopsies were also graded for inflammation and fibrosis. 【Results】 A significant elevation in the expressions of SREBP-1C and GRP-94 in liver of NAFLD group were found compared to the control group（P〈0.01）. 【Conclusion】 The excessive expressions of SREBP-1C and GRP-94 could participate in the development of nonalcoholic fatty liver. This information suggested that was involved in mechanism of nonalcoholic fatty liver.

关 键 词：非酒精性脂肪肝 固醇调节元件结合蛋白-1C 糖调节蛋白94 

分 类 号：R575.5[医药卫生—消化系统]