剑麻总皂苷高产菌的筛选及发酵条件的优化  

Screening High-Yielding Strain for Extracting Agave sisalana perr.ex Engelm Total Saponins and Its Fermentation Condition

作　　者：周菲菲[1] 郝再彬[1] 易克贤[2] 郑金龙[2] 王秀丽[1] 

机构地区：[1]桂林理工大学化学与生物工程学院,广西桂林541004 [2]中国热带农业科学院环境与植物保护研究所,海口571101

出　　处：《湖北农业科学》2014年第8期1863-1866,1911,共5页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(31060193;31200269);国家麻类产业技术体系建设专项(CARS-19)

摘　　要：以绿色环保发酵生产提取剑麻(Agave sisalana perr.ex Engelm)总皂苷为目标,采集土壤样品,对菌株进行初筛和复筛得到1株菌株,编号JMZ-N06。通过料液比、发酵温度、发酵时间、发酵液初始pH四因素三水平的正交试验,确定了该菌的最适发酵条件为料液比1∶20(g∶mL),发酵时间60 h,发酵温度32℃,发酵液初始pH 6.0。To environmentally ectract total saponins from Agave sisalana perr.ex Engelm , one strain of No. JMZ-N06 was obtained after twice screen from hundreds of soil samples. The optimal fermentation conditions were determined by orthogonal test of three levels and four factors including ratio of material to liquid, fermentation temperature, fermentation time, initial pH in fermentation broth. The results showed that the yield of total saponins from Agave sisalana perr.ex Engelm was 142.5 mg∕g when fermented 60 h under 32℃ with the material to liquid ratio of 1：20（g：mL） and initial pH of 6.0.

关 键 词：剑麻(Agave sisalana perr EX Engelm) 正交试验 发酵条件 优化 

分 类 号：Q815[生物学—生物工程]