棕榈酸促进肝癌细胞侵袭转移的分子机制  

Molecular Mechanism of Palmitic Acid Induced Invasion and Metastasis of Hepatoma Carcinoma Cells

作　　者：刘璐[1] 张先娇[2] 权晓娟[3] 罗苗[1] 张磊[1] 徐孝娜[1] 王枫[1] 刘寒强[1] 

机构地区：[1]第四军医大学军事预防医学系营养与食品卫生学教研室,陕西西安710032 [2]陕西省人民医院医学教育科研处,陕西西安710068 [3]西安交通大学第二附属医院老年病科,陕西西安710004

出　　处：《现代生物医学进展》2014年第22期4223-4227,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金面上项目(81072303)

摘　　要：目的:探讨棕榈酸(Palmiticacid,PA)对人肝癌细胞系SMMC-7721侵袭转移能力的影响,并通过检测肝癌细胞系中CD147-MMPs信号通路在PA影响下的变化,初探PA影响肝癌细胞侵袭转移的分子机制。方法:PA(0、20、50、100μM)作用SMMC-7721细胞后(8、16、24h),MTT法检测细胞增殖,划痕及Transwell实验评价细胞迁移侵袭能力,Western-blot及real-time PCR检测CD147蛋白及其mRNA的水平,ELISA检测基质金属蛋白酶(MMP-2,MMP-9)的水平。结果:与对照组相比,PA作用SMMC-7721细胞后,细胞存活率无显著差异(P>0.05);细胞迁移和侵袭能力显著增高(P<0.05);CD147蛋白及其mRNA的表达显著增高(P<0.05);培养上清中MMP-9的浓度显著增高(P<0.05),MMP-2的水平则无变化。不同的梯度组之间相比较,细胞迁移和侵袭能力、CD147的表达水平(蛋白及其mRNA)以及培养上清中MMP-9的浓度均随PA作用时间和作用剂量的增大而产生更显著的增高。结论:PA通过活化CD147-MMPs信号通路促进SMMC-7721细胞的迁移侵袭。Objective： The impact of dietary fatty acids on the development and progression of cancer is highly controversial. In this study, we investigated whether palmitic acid（PA, saturated fatty acids） enhances migration and invasion of hepatoma cells via a CD147-MMPs pathway. Methods： Human SMMC-7721 cells was exposed to PA. MTT assay was used for cell viability and wound healing, transwell assays for cell migratory and invasive abilities, qRT-PCR for CD147 mRNA expression, Western blot for CD147 protein expression, and ELISA for MMPs levels. Results： Compared to control, PA has no significant effect on cell growth; promotes the migration and invasion of cells; induces a up-regulation of CD147 expression and stimulates cells to secrete MMP-9.Within a certain concentration range（0,20,50,100μM）, PA can enhances the migration and invasion of cells, up-regulates CD147 expression（protein and mRNA level） and stimulates MMP-9 secretion in a dose- and time-dependent manner. Conclusion： Taken together, our findings demonstrate that PA has potential to promote the migration and invasion of SMMC-7721 cells through the activation of CD147-MMPs pathway in vitro.

关 键 词：棕榈酸 肝癌 侵袭转移 CD147 基质金属蛋白酶 

分 类 号：R735.7[医药卫生—肿瘤]