冬凌草甲素对人结肠癌细胞株HCT116生长的影响及其机制研究  

Effects and Mechanisms of Oridonin on the Growth of HCT116 Cells

作　　者：金星镜[1] 丁文评[1] 张莲[1] 田玮[1] 陈思宇[1] 

机构地区：[1]上海交通大学医学院附属新华医院肿瘤科,上海200092

出　　处：《现代生物医学进展》2014年第23期4444-4446,4450,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81274142;30300139);上海市科委自然基金项目(11ZR1423400);上海市教委重点项目(07ZZ43)

摘　　要：目的:研究冬凌草甲素(ORI)对人结肠癌细胞株HCT116生长的影响及其可能机制。方法:以体外培养的HCT116细胞为研究对象,给予不同浓度(0、2.5、5、10、20μM)ORI处理HCT116细胞不同时间(0、24、48、72 h),通过MTT法检测其对HCT116细胞增殖的影响,DAPI染色观察其对细胞核的形态的影响,western blot检测细胞内β-catenin、c-myc蛋白表达的变化。结果:1ORI可显著抑制HCT116细胞的增殖,且此作用随着浓度和作用时间的增加或延长而增强(P<0.05)。2ORI处理HCT116细胞24小时后,细胞核固缩的百分率随药物作用浓度的增加而增加。35、10、20μM ORI处理HCT116细胞24小时后,细胞内的β-catenin、c-myc蛋白水平均显著下调,且随着ORI浓度的增加逐渐减少。结论:ORI能以浓度和时间依赖性的方式抑制HCT116细胞的增殖,其机制可能与Wnt/β-catenin信号通路有关。Objective： To investigate the effect and mechanisms of oridonin on the growth of human colon cancer HCT116 cells.Methods： HCT116 cells were cultured in vitro and treated with various concentrations（0, 2.5, 5, 10, 20 μM） of ORI for different durations（0, 24, 48, 72 h）. The cytotoxic effect of ORI on HCT116 cells was observed by MTT assay. The morphological nuclear changes following treatment with ORI（0, 10, 20 μM） for 24 h were observed under the fluorescence microscope. Western Blot was used to investigate the expression levels of β-catenin, c-myc in HCT116 cells. Results： 1 HCT116 proliferation was inhibited in a time and concentration dependent manner after treatment of ORI（P〈0.05）. 2With the increase of ORI concentration, the percentage of nuclear condensation increased. 3 The expression of β-catenin and c-myc both significantly down-regulated with the ORI concentration increased. Conclusion： ORI could inhibit the HCT116 cells proliferation in a time and concentration dependent manner, and the mechanism may be associated with the Wnt/β-catenin signal pathway.

关 键 词：冬凌草甲素 结肠癌 HCT116细胞 Β-CATENIN C-MYC 

分 类 号：R733[医药卫生—肿瘤]