MYCN转基因型斑马鱼系岩藻糖转移酶基因谱表达的研究  

Study of the Gene Expression of Fucosyltransferases in MYCN Transgenic Zebrafish/Danio Rerio

作　　者：花紫菱[1] 沈莉菁[2] 李丽玮[1] 李志强[1] 

机构地区：[1]上海交通大学附属第六人民医院,200233 [2]上海交通大学附属仁济医院血液科

出　　处：《临床输血与检验》2014年第3期273-276,共4页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的研究岩藻糖转移酶在髓系白血病的发生与发展中的作用。方法应用实时定量逆转录-聚合酶链反应检测具髓系白血病表型的MYCN转基因型斑马鱼系岩藻糖转移酶基因(DFs)表达。结果 MYCN转基因型斑马鱼系岩藻糖转移酶7/8/9/10/11(DF7/8/9/10/11)表达量与野生型斑马鱼相比显著改变(t=-32.756、8.405、12.719、34.673、27.113,P<0.05);岩藻糖转移酶1/2(DF1、2)表达量的差异无统计学意义(t=-1.651、-0.244,P>0.05)。结论在MYCN转基因型斑马鱼系中,岩藻糖转移酶的表达与髓系白血病的发生与发展存在相关性。Objective To investigate the function of fucosyltransferases in myeloid leukemia.Methods Using realtime reverse transcription polymerase chain reaction（RT-PCR）to detect the relative quantitation of FUTs mRNA expression in MYCN transgenic Zebrafish/Danio Rerio which possesses the characteristics of acute myeloid leukemia.Results The expressions of DF7/8/9/10/11in the MYCN transgenic Zebrafish/Danio Rerio altered significantly,comparal to the wild type（WT）fish（t=-32.756,8.405,12.719,34.673,27.113,P〈0.05）.As to the expressions of DF1/2in the MYCN transgenic Zebrafish/Danio Rerio,there were no statistical significance between the two germ lines（t=-1.651,-0.244,P〉0.05）.Conclusion The mRNA expressions of DFs were relevant to the occurrence and development of myeloid leukemia in the MYCN transgenic Zebrafish/Danio Rerio.

关 键 词：髓系白血病 岩藻糖转移酶 斑马鱼 

分 类 号：R733.7[医药卫生—肿瘤] R392.11[医药卫生—临床医学]