响应面法优化鹰嘴豆铁蛋白提取工艺  

Optimization of Extraction Process of Ferritin from Cicer arietinum by Response Surface Methodology

作　　者：辛敏[1,2] 黄昀[1] 詹欣[1,2] 王然[1,2] 唐劲天[1,2] 

机构地区：[1]清华大学工程物理系粒子技术与辐射成像教育部重点实验室,北京100084 [2]北京中医药大学中药学院,北京100102

出　　处：《中国实验方剂学杂志》2014年第14期12-16,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金青年基金项目(81102889)

摘　　要：目的:优选鹰嘴豆铁蛋白的提取工艺。方法:在单因素试验基础上,选择缓冲液pH、料液比、盐析盐摩尔浓度为自变量,干膏量及铁蛋白、总蛋白得率为响应值,根据Box-Behnken原理采用三因素三水平响应面分析法优选鹰嘴豆铁蛋白的提取工艺参数。结果:最佳提取工艺条件为缓冲液pH 7.46,料液比1∶4,盐析盐摩尔浓度50 mmol·L-1;铁蛋白得率0.002 556%,与理论预测值0.002 633%偏差较小。结论:采用响应面法优选的鹰嘴豆铁蛋白提取工艺稳定合理,为提高铁蛋白得率和植物补铁制剂的开发利用提供参考。Objective： To optimize extraction process of ferritin from Cicer arietinum. Method： On the basis of single factor tests, response surface methodology （RSM） was applied for optimizing extraction conditions with solid-liquid ratio, buffer pH, salt concentration as independent variables and yields of ferritin and total proteins, dry weight of extract as response value. According to Box-Behnken principles, RSM with three factors and three levels was used. Result： Optimum extraction conditions for ferritin from C. arietinum was as follows： buffer pH of 7.46, solid-liquid ratio of 1 ： 4, molar concentration of MgC12 50 mmol·L^-1 ; yield of ferritin was 0. 002 556% , whose deviation was small with theoretical predicted value of 0. 002 633%. Conclusion： RSM for optimizing extraction process of ferritin was stable and feasible, it could improve extraction conditions of ferritin and provide a reference for development and utilization of plant iron supplements.

关 键 词：鹰嘴豆 铁蛋白 响应面分析法 提取工艺 邻菲咯啉显色法 

分 类 号：R283.6[医药卫生—中药学]