不同剂量左甲状腺素对缺氧缺血性脑损伤新生大鼠脑组织HIF-1α表达的调节  

Role of L-thyroxine on brain tissue hypoxia-inducible factor 1α expression after hypoxia-ischemia brain damage in neonatal rats

作　　者：张晓莉[1] 贾天明[1] 杜开先[1] 赵闪闪[1] 牛国辉[1] 

机构地区：[1]郑州大学第三附属医院儿内科,河南郑州450052

出　　处：《中国儿童保健杂志》2014年第7期702-704,708,共4页Chinese Journal of Child Health Care

基　　金：2013年河南省教育厅自然科学研究计划项目(13A320664)

摘　　要：目的探讨不同剂量左甲状腺素(L-T4)对缺氧缺血性脑损伤(hypoxia-ischemia brain damage,HIBD)新生大鼠脑组织缺氧诱导因子-1α(hypoxia-inducible factor 1α,HIF-1α)的调节作用。方法 7日龄SD大鼠随机分为假手术组、缺氧缺血组(HI)、溶剂组及L-T4低、高剂量组。低、高剂量L-T4干预组及溶剂对照组分别于HIBD后当日起腹腔注射2μg/100g L-T4、3.5μg/100g L-T4及等体积溶剂,每日1次,共5d。应用免疫组化法检测鼠脑HIF-1α蛋白,RT-PCR法检测HIF-1αmRNA。结果缺氧缺血组缺血侧大脑皮质HIF-1α蛋白(72.795±6.121)及HIF-1αmRNA(0.448±0.035)表达较假手术组(依次为38.581±2.846,0.174±0.015)增高,差异有统计学意义(P<0.05);L-T4低、高剂量干预组缺血侧大脑皮质HIF-1α蛋白(依次为117.350±9.374,142.842±8.948)及HIF-1αmRNA(依次为0.618±0.042,0.711±0.049)表达较缺氧缺血组增高,差异有统计学意义(P<0.05),且L-T4对HIF-1α的影响具有剂量依赖性,即随剂量增加,HIF-1α蛋白及HIF-1αmRNA表达增加,两组间差异具有统计学意义(P<0.05)。结论在新生大鼠HIBD时,L-T4可上调HIF-1α蛋白及其mRNA表达,从而对HIBD起保护作用,且具有剂量依赖性。Objective To observe the expression of hypoxia-inducible factor la（HIF 1-） in rat brain after hypoxia-is chemia brain damage （HIBD） and L-thyroxine （L-T4） of different dose treated. Methods Eighty postnatal 7 days old Sprague-Dawley（SD） rats were randomly divided into 5 groups ：the sham operation group, hypoxia-ischemia group （HI）, menstruum-treated group, L-T4-treated groups of low and high dose. The rats of menstruum-treated, L-T4 treated groups of low and high dose were respectively daily administrated of intraperitoneal injection of menstruum of the equal volume and 2 μg/100 g L-T4,3.5μg/100 g,once a day,for 5 days. The expression of HIF-lα were detected by immunohistochemistry. Re- verse transcription-polymerase chain reaction（RT-PCR） was used to detect the level of HIF-1α mRNA. Results Com- pared with the sham operation group,the levels of HIF-1α protein（38. 581±2. 846） and HIF-1α mRNA（0. 174±0. 015） were upregulated in HI group（72. 795±. 121,0. 448±0. 035 respectively） ,as welt as in menstruum-treated group. The lev- els of H1F-1α protein （117. 350±9. 374,142. 842±8. 948 respectively）and HIF-1α mRNA（0. 618±0. 042,0. 711±0. 049 respectively） in L-T4-treated groups of low and high dose were higher than HI group（P〈0.05）. The influence of L T4 on HIF-1α protein and HIF-1α mRNA was dose dependent, namely with the increase of dose, their expression increased （P〈 0.05）. Conclusion L-T4 upregulats HIF-1α mRNA and protein expression in neonatal rats with HIBD.

关 键 词：缺氧诱导因子1-Α 左甲状腺素 缺氧缺血性脑损伤 新生大鼠 

分 类 号：Q95-33[生物学—动物学]