缺氧诱导因子1α和P糖蛋白在乳腺癌中的表达及意义  

Expression and significance of hypoxia-inducible factor-1α and P-glycoprotein in breast cancer

作　　者：王芳[1] 付占昭[1] 张丽娇[1] 韩惠萍 

机构地区：[1]秦皇岛市第一医院肿瘤科,河北秦皇岛066000

出　　处：《临床荟萃》2014年第7期744-746,F0002,共4页Clinical Focus

摘　　要：目的检测缺氧诱导因子1α(HIF-1α)和P糖蛋白(P-gp)在乳腺癌组织中的表达并分析其临床病理学意义及两者之间的相关性。方法采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶连结法(SP法)检测59例乳腺癌组织和15例癌旁正常乳腺组织中HIF-1α和P-gp的表达。结果 HIF-1α和P-gp在乳腺癌组织中的阳性表达率分别为61.0%(36/59)、40.7%(24/59),均明显高于正常乳腺组织(P<0.05)。HIF-1α和P-gp在有淋巴结转移组中的阳性表达率均明显高于其在无淋巴结转移组中的阳性表达率(76.5%vs 40.0%和52.9%vs 24.0%),差异具有统计学意义(P<0.05)。随着乳腺癌肿块的增大、组织学分级的增加,HIF-1α的阳性表达率均增高(分别为30.8%、65.7%、81.8%和22.2%、58.6%、81.0%),并均具有线性趋势(P<0.05),而P-gp阳性表达率的差异均无统计学意义(P>0.05)。在不同年龄、病理类型及月经状态组中HIF-1α和P-gp阳性表达率的差异均无统计学意义(P>0.05)。HIF-1α与P-gp表达呈正相关表达(r=0.380,P<0.05)。结论 HIF-1α和P-gp的高表达提示乳腺癌具有更高的侵袭性,可联合检测作为判断乳腺癌恶性程度及临床化疗疗效的指标。Objective To evaluate the expression of hypoxia-inducible factor-1α（HIF-1α）and P-glycoprotein（Pgp）in breast cancer and investigate the relationships between their expressions and the clinicopathological characteristics,and also analyze the association among their expressions.Methods The expression of HIF-1αand P-gp in 59breast cancer samples and 15normal breast tissues were detected using immunohistochemical SP methods.Results The positive expression of HIF-1αand P-gp in 59cases were 61.0%（36/59）and 40.7%（24/59）respectively,and there were significant differences between breast cancer tissues and normal tissues（P〈 0.05）.Both HIF-1αand P-gp expression had significantly positive correlation with lymph node metastasis（P〈0.05）.The expression of HIF-1αwas positively related to tumor size and histological grade,while P-gp expression was not.Neither of them was correlated with the patients＇ age,histological type and menstruation status（P〉0.05）.Significant correlation existed between the expression of HIF-1αand P-gp in breast cancer（r=0.38,P〈0.05）.Conclusion The over expression of HIF-1αand P-gp showed that the invasion of breast cancer is even higher.Combined detection of the two indicators can be used to accurately assess the prognosis in breast cancer and evaluate the clinical effects of chemotherapy.

关 键 词：乳腺肿瘤 免疫组织化学 缺氧诱导因子-1Α P-糖蛋白 

分 类 号：R737.9[医药卫生—肿瘤]