Akt1在环氧合酶-2抑制剂抑制宫颈癌Hela细胞增殖中的作用  

Effect of Akt1 on Inhibition of Cervical Cance Hela Cell's Proliferation by Cyclooxygenase-2 Inhibitor

作　　者：刘小叶[1] 周建斌[1] 李婷[1] 黄余良[1] 

机构地区：[1]南华大学附属第二医院妇产科,湖南衡阳421001

出　　处：《中南医学科学杂志》2014年第4期359-362,共4页Medical Science Journal of Central South China

摘　　要：目的研究Akt1在环氧合酶2抑制剂NS398抑制宫颈癌Hela细胞增殖过程中的作用。方法以不同浓度的NS398或Akt1选择性激动剂IGF-1与NS398共同处理Hela细胞,采用噻唑蓝(MTT)比色法分析细胞生长抑制率,Western blotting免疫印迹技术分别检测Akt1蛋白表达的变化。结果与对照组比较,不同浓度的NS398处理Hela细胞后,细胞生长抑制率(IR)增加(P<0.05);NS398呈浓度和时间依赖性下调Hela细胞Akt1蛋白质的表达(P<0.05);与单独用NS398组比较,Akt1激动剂与NS398共同处理细胞后,细胞IR值逐渐下降,并且其作用呈浓度依赖和时间依赖性(P<0.05)。结论 NS398呈剂量和时间依赖性抑制Hela细胞的增殖,其作用机制可能与下调Akt1蛋白的表达有关。Objective Investigate to the effect of Akt1 on Inhibition of Cervical Cance Hela Cell Proliferation by Cyclooxygenase-2 inhibitor. Methods Hela cells were treated with NS398 and（ or） Akt1 agonist IGF-1,then the growth inhibitory rate（ IR） of cell was evaluated by MTT assay. The expressions of Akt1 were detected by Western Blotting. Results NS398 significantly increase the IR of Hela cells and down-regulate the Akt1 protein expression（ P〈 0. 05）. Akt1agonist IGF-1 decreased the IR of Hela cells compared with only treated with NS398 groups. These effects are dose-dependent manner. Conclusion NS398 inhibits Hela cell growth in a dose-dependent and time-dependent manners,and the mechanism may be related to down-regulate the expressions of Akt1.

关 键 词：环氧合酶2抑制剂 宫颈癌 蛋白激酶B 细胞增殖 

分 类 号：R737.33[医药卫生—肿瘤]