转录因子Twist在胰腺癌中的表达及在上皮间质转化中的作用  

Expression of Twist in pancreatic carcinoma and its role in epithelial mesenchymal transition

作　　者：张同军[1] 薛栋[1] 刘敏[2] 李新军[3] 张成德[1] 成丕光[1] 巩本刚[1] 

机构地区：[1]滨州市人民医院肝胆外科,滨州256610 [2]滨州市人民医院干部保健二科,滨州256610 [3]滨州市人民医院病理科,滨州256610

出　　处：《国际外科学杂志》2014年第6期382-386,F0003,共6页International Journal of Surgery

摘　　要：目的 探讨Twist介导的上皮间质转化在胰腺癌进展中的作用.方法 采用免疫组织化学法检测42例胰腺癌组织中Twist、N-cadherin及Vimentin蛋白的表达,26例癌旁组织作为对照.结果 胰腺癌组织中Twist、N-cadherin及Vimentin蛋白的阳性表达率分别为76.2％、59.5％和57.1％.Twist、N-cadherin 及Vimentin在26例癌组织及配对癌旁组织中的阳性表达率比较,差异均有统计学意义（均P＜0.001）;Twist、N-cadherin及Vimentin的表达均与肿瘤分化程度、TNM分期、神经浸润及淋巴结转移明显相关（P＜0.05）.经Spearman等级相关性分析,Twist与N-cadherin及Vimentin在胰腺癌组织中的表达均呈正相关（r =0.506,P=0.001;r=0.417,P=0.006）;N-cadherin及Vimentin在胰腺癌组织中的表达呈正相关（r=0.462,P=0.002）.结论 Twist可能通过诱导胰腺上皮细胞发生上皮间质转化促进胰腺癌侵袭转移.Objective To investigate the relationship of Twist induced epithelial mesenchymal transitions （EMT） during pancreatic carcinoma progression.Methods The expressions of Twist,N-cadherin and Vimentin proteins were tested by immunohistochemistry in 42 cases of pancreatic carcinomas and 26 cases of adjacent paracancerous tissues.Results The positive rates of Twist,N-cadherin and Vimentin in 42 cases of pancreatic carcinomas were 76.2％,59.5％ and 57.1％,respectively.The positive rate of Twist,N-cadherin and Vimentin were all significant between pancreatic carcinomas and paired tumor-adjacent paracancerous tissues （all P ＜0.001）.The differences of the expression of Twist,N-cadherin and Vimentin in pancreatic carcinoma of tumor differentiation,TNM stage,neural invasion and lymph node metastasis were significant （all P ＜ 0.05）.Significantly positive correlation was found between the expression of Twist and the others （N-cadherin and Vimentin） by using spearman correlation analysis （r =0.506,P =0.001 ; r =0.417,P =0.006）.Significantly positive correlation was found between the expression of N-cadherin and Vimentin by using spearman correlation analysis（r =0.462,P =0.002）.Conclusions Twist signaling pathway activation might mediate pancreatic epithelial cells to EMT and then promote pancreatic carcinoma invasion and metastasis.

关 键 词：胰腺肿瘤 TWIST转录因子 N-钙黏素 波形蛋白 上皮间质转化 免疫组织化学 

分 类 号：R735.9[医药卫生—肿瘤]