微小RNA-10b对胶质瘤细胞增殖能力的影响  

Effect of microRNA-lOb on proliferation of gfloma cells

作　　者：孙利波[1] 刘莉[2] 梁华新[1] 付超[1] 赵丛海[1] 

机构地区：[1]吉林大学中日联谊医院神经外一科,长春130033 [2]吉林大学白求恩医学部法医教研室

出　　处：《中华实验外科杂志》2014年第7期1514-1516,共3页Chinese Journal of Experimental Surgery

基　　金：吉林省科技发展计划资助项目（201201045）

摘　　要：目的 观察微小RNA-10b（miR-10b）在胶质瘤中的表达及其对胶质瘤细胞增殖能力的影响。方法 利用TaqMan？实时定量聚合酶链反应（Real-time PCR）法检测77例不同级别胶质瘤组织、15例正常脑组织及胶质瘤细胞株中miR-10b的表达。采用噻唑蓝（MTT）比色法及流式细胞仪检测转染miR-10b模拟物对胶质瘤A172细胞增殖能力及细胞周期分布的影响。结果 miR-10b在胶质瘤细胞和胶质瘤组织中的表达水平明显高于正常脑组织（4.46±0.24比0.44±0.10,P〈0.01）,且miR-10b的表达水平与胶质瘤组织病理分级呈正相关（I级：2.10±0.27,Ⅱ级：3.58±0.35、Ⅲ级：4.85±0.38、Ⅳ级：6.38±0.37,P〈0.01）。过表达miR-10b能够促进A172细胞的体外增殖能力,并使A172细胞处于G0/G1期的细胞减少,进入S期的细胞明显增多。结论 miR-10b在胶质瘤中过表达,加速胶质瘤细胞进入S期,促进增殖。Objective To investigate the effect of microRNA （miR）-10b expression on prolifera- tion of glioma cells. Methods miR-10b expression levels in glioma cell line, 77 cases of giloma tissues and 15 normal brain tissues were detected by TaqMang real-time polymerase chain reaction （PCR）. miR-10b mimics were transfected into A172 cells using Lipofectamine 2000 reagent. After up-regulating the miR-10b expression, cell growth ability in vitro was determined by dimethyl thiazolyl diphenyl tetrazolium （MTT） assay and the cell-cycle distribution was analyzed using flow cytometry. Results The expression of miR-10b in both glioma tissues and glioma cell line was significantly higher than that in normal brain tis- sues （4.46 ±0.24 vs. 0.44 ±0. 10,P〈0.01）. There was a positive correlation between the level of miR-10b expression and grades of glioma tissues （2. 10 ± 0. 27 for grade 1, 3.58 ± 0. 35 for grade Ⅱ , 4. 85 ±0. 38 for grade Ⅲ, and 6. 38 ±0. 37 for grade Ⅳ ,P 〈0. 01 ）. Ectopic over-expression of miR-10b promoted the proliferation of Al72 cells and increased the percentage of A172 cells in S phase. Conclusion miR-10b was up-regulated in glioma and promoted the proliferation of glioma cells.

关 键 词：微小RNA-10b 胶质瘤 增殖 

分 类 号：R739.41[医药卫生—肿瘤]