过氧化物酶体增殖物激活受体γ拮抗剂抑制甲状腺相关眼病眼眶前脂肪细胞分化的研究  

Experimental research on preadipocyte differentiation inhibition by peroxisome proliferator activated receptor γ antagonist

作　　者：王莉菲 刘静江 闫忠阳 

机构地区：[1]河北省眼科医院,河北省眼科学重点实验室,河北邢台054001

出　　处：《中华实验外科杂志》2014年第7期1540-1542,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 探讨过氧化物酶体增殖物激活受体γ（PPARγ）拮抗剂GW9662在眼眶前脂肪细胞分化过程中的抑制作用。方法 培养并分化8例甲状腺相关眼病（TAO）患者眼眶脂肪组织前脂肪细胞,按GW9662浓度不同将实验分为7组。进行不同处理条件下的前脂肪细胞分化。测量各组细胞内脂肪含量改变、脂肪细胞分化率及PPARγmRNA表达的改变。结果 10、50μmol/L GW9662组脂肪含量最高吸光度（A）值分别为0.406±0.010及0.296±0.034,较其他组差异有统计学意义（P〈0.05）。随GW9662浓度的增加,脂肪细胞分化率分别为11.9%、1O.0%、6.7%、4.1%,激动剂组增长至61.3%,GW9662拮抗后降至21.2%。10μmol/L及50μmol/L GW9662作用下PPARγ表达明显减弱,相对灰度值分别为0.51±0.06和0.39±0.05,较其他组差异有统计学意义（P〈0.005）。结论 GW9662可随剂量的增加降低脂肪细胞的分化率,降低细胞PPARγ的表达,从而证明GW9662具有抑制脂肪细胞分化的能力。Objective To observe the function of peroxisome proliferator activated receptor γ （PPARγ） antagonist GW9662 on inhibiting preadipoeyte differentiation. Methods The human orbital preadipocytes of 8 throid-assoeiated ophthalmopathy （TAO） patients were cultured. According to the concentration of GW9662, seven groups were divided. Preadipocyte differentiation under different conditions was processed. The fat contents changes, rate of the fraction cells and PPAR3, genes changes were investigated. Results The highest A value of fat content in 10 μmol/L GW9662 and with 50μmol/L GW9662 groups were 0. 406 ± 0. 010 and 0. 296 ±0. 034. There was statistically significant difference compared with other groups （ P 〈 0.05 ）. The adipocyte differentiation rate gradually decreased followed the increased concentration of GW9662, which were 11.9% , 10.0%, 6. 7% and 4. 1%, while the rate increased up to 61.3% under the function of 10 μmol/L rosiglitazone. The expression of PPAR.become weak after treated with 10 μmol/L and 50 μmol/L GW9662. Conclusion GW9662 can severely reduced adipogenesis and the expression of adipose-related genes.

关 键 词：甲状腺相关眼病 脂肪分化 过氧化物酶体增殖体激活受体γ拮抗剂 

分 类 号：R771.3[医药卫生—眼科]