HTR3E基因多态性与中国女性IBS-D的发生及肠道症状的关联  

Association between HTR3E gene polymorphism and intestinal symptoms of diarrhea-predominant irritable bowel syndrome in Chinese women

作　　者：张瑜[1] 廖月霞[1] 陶文华[1] 李瑶瑶[1] 郝臻凤[1] 卜平[1] 

机构地区：[1]扬州大学医学院中西医结合研究所,江苏省扬州市2005001

出　　处：《世界华人消化杂志》2014年第17期2503-2507,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81173392;江苏省研究生创新工程基金资助项目;No.CXZZ12_0918~~

摘　　要：目的:探讨五羟色胺受体3(5-hydroxytryptamine-3 receptor,5-HTR3)的亚型HTR3E基因非翻译区多态性与中国女性IBS-D的发生及肠道症状的关联.方法:采用聚合酶链反应-限制性片段长度多态性分析(polymerase chain reactionrestriction fragment length polymorphism,P C R-R F L P)对294例I B S-D女性患者与300例健康对照者HTR3E基因3'端非翻译区c.*76G>A(rs62625044)进行研究.结果:女性患者c.*76G>A多态性位点GA基因型(χ2=6.362,P=0.012)和A等位基因(χ2=5.970,P=0.015)的频率较正常对照均显著升高(P<0.05),GA基因型患者较GG基因型排便频率更高(χ2=7.68,P=0.021),两组患者排便性状也存在很大差异(χ2=6.225,P=0.044).结论:HTR3E的GA基因型和A等位基因可能是中国IBS-D女性患者的易感因素之一;GA基因型的患者较GG基因型肠道症状更加严重.AIM： To investigate the relationship between 5-hydroxytryptamine-3 E receptor （HTR3E） gene polymorphism and intestinal symptoms of diarrhea-predominant irritable bowel syndrome （IBS-D） in Chinese women. METHODS： Polymerase chain reaction （PCR）-restriction fragment length polymorphism （RFLP） technique was used to detect the 3＇UTR variant c.＊76G〉A of the HTR3E gene in 300 healthy female subjects and 294 female IBS-D patients.RESULTS： There were significant differences between the IBS-D patients and controls in the frequencies of the GA genotype （χ2 = 6.362, P = 0.012） and the A allele （χ2 = 5.970, P = 0.015）. Compared with the GG genotype of HTR3E, the patients with GA genotype had a higher frequency of defecation （χ2 = 7.68, P = 0.021） and more serious stool form （χ2= 6.225, P = 0.044）. CONCLUSION： The GA genetype of HTR3E might be an important predisposing factor for IBS-D in Chinese females and might contribute to the deterioration of the intestinal symptoms.

关 键 词：腹泻型肠易激综合征 HTR3E 多态性 肠道症状 

分 类 号：R574[医药卫生—消化系统]