酶解竹子溶解浆制备纳米微晶纤维素的研究  

Study on Preparation of Nano-crystalline Cellulose from Enzymolysis of Bamboo Dissolving Pulp

作　　者：卓治非[1,2,3,4] 房桂干[1,2,3,4,5] 王戈 沈葵忠[1,2,3,4,5] 邓拥军[1,2,3,4,5] 卢婷婷[1,2,3,4] 

机构地区：[1]中国林业科学研究院林产化学工业研究所 [2]江苏省生物质能源与材料重点实验室 [3]国家林业局林产化学工程重点开放性实验室 [4]生物质化学利用国家工程实验室,江苏南京210042 [5]中国林业科学研究院林业新技术研究所,北京100091 [6]国际竹藤中心,北京100102

出　　处：《造纸科学与技术》2014年第3期6-8,71,共4页Paper Science & Technology

基　　金："十二五"国家科技支撑计划项目(2012BAD23B0202)

摘　　要：纳米微晶纤维素(NCC)可由可再生资源制备,并且具有诸多特性,近年来成为研究热点。本文应用PFI磨对竹子溶解浆预处理,用纤维素酶水解制备纳米微晶纤维素,研究了酶解时间、酶解温度、酶用量对纳米微晶纤维素产率的影响,采用正交实验优化了工艺参数。并用高效液相色谱仪、马尔文激光粒度仪对水解液及NCC进行表征。结果表明:在酶用量2.736FPU/g、酶解时间3d、酶解温度50℃的条件下,纳米微晶纤维素的产率最高,达到19.13%;高效液相色谱分析表明水解液主要成分为葡萄糖,占总还原糖含量的71.06%,其次为纤维二糖12.39%,木糖7.68%;激光粒度分析表明NCC的Z均粒径为375.5nm。Nanocrystalline cellulose （NCC）has attracted a significant interest in recent years as it is made from re- newable resources and has many unique characteristics. In this paper, bamboo dissolving pulp was preproeessed by PFI mill, and nano-crystalline cellulose was prepared from bamboo dissolving pulp with cellulase hydrolysis. The effects of time, temperature of enzymolysis and dosage of enzyme on the yield of NCC were studied which have an influence on the productivity of nano-crystalline cellulose. The process parameters were optimized by orthogonal ex- periment. Using scanning electron microscope, laser particle sizer, fourier transformed infrared spectroscopy, ther- mogravimetric, the raw material and NCC were proceeded properties characterization. The result shows： the pro- ductivity of nano-crystalline cellulose is the highest（ 19. 13% ）at the condition of 2. 736FPU dosage of enzyme, 3d enzymolysis time, 50℃ enzymolysis temperature ; HPLC （High Performance Liquid Chromatography）analysis shows that the main element of hydrolysate is glucose, which is 71.06% of total reducing sugar, secondly, 12. 39% cel- lobiose, 7.68% xylose; laser particle size analysis shows Z-average size of NCC is 375.5nm.

关 键 词：纤维素酶 竹子溶解浆 纳米微晶纤维素 性能表征 

分 类 号：TB383[一般工业技术—材料科学与工程]