宫颈正常细胞与ASC-US中HPV感染型别的对比研究  

A comparative study of HPV infective genotypes in normal cells and ASC-US in uterine cervix

作　　者：梁晓东[1] 蔡为民[2] 耿建祥[2] 龙秀荣[2] 张劲松[2] 王宏景[2] 夏林[2] 李海[2] 赵雪[2] 

机构地区：[1]江苏省盐城市第三人民医院病理科,江苏盐城224001 [2]南京中医药大学第三附属医院病理科/江苏省HPV协作组,江苏南京210001

出　　处：《国际检验医学杂志》2014年第13期1699-1701,1703,共4页International Journal of Laboratory Medicine

基　　金：南京市卫生局中医专项资助项目(2009-92)

摘　　要：目的比较女性宫颈正常细胞与不典型鳞状细胞(atypical squamous cells of undetermined significance,ASC-US)中人乳头瘤病毒(human papillomavirus,HPV)感染的基因型分布情况及其临床意义。方法采用基因扩增结合基因芯片技术对1 000例宫颈正常细胞和229例宫颈ASC-US标本进行23种HPV基因分型检测。结果 1 000例宫颈正常细胞中检出HPV阳性感染者106例,总的HPV感染率为10.60%(106/1 000),其一重感染率为9.30%(93/1 000),多重感染率为1.30%(13/1 000);229例宫颈ASC-US中检出HPV阳性感染者116例,总的HPV感染率为50.66%(116/229),其一重感染率为34.06%(78/229),多重感染率为16.59%(38/229)。两组HPV总阳性率、一重和多重感染率比较差异有统计学意义(P<0.05)。结论 HPV16、18、33、42、43、52、58型是宫颈正常细胞及ASC-US中最主要的型别,基因扩增结合基因芯片检测技术可应用于宫颈细胞的HPV分型检测,有利于在临床上对宫颈ASC-US行进一步分流管理,对宫颈癌的防控具有重要意义。Objective To compare the distribution situation of human papillomavirus（HPV）infective genotypes in normal cells and atypical squamous cells of undetermined significance（ASC-US）in uterine cervix and its clinical significance.Methods The pol-ymerase chain reaction（PCR）combined with the gene-chips technology were adopted to detect 23 kinds of HPV genotype from 1 000 cases of normal cells specimens and 229 cases of ASC-US specimens.Results 106 cases of HPV-positive infection were de-tected from 1 000 cases of normal cells with the total HPV infection rate of 10.60%（106/1 000）,in which the single genotype in-fection rate was 9.30%（93/1 000）and the multiple genotypes infection rate was 1.30%（13/1 000）;116 cases of HPV-positive in-fection were detected from 229 cases of cervial ASC-US specimens with the total HPV positive rate was 50.66% （116/229 ）,in which the single genotype infection rate was 34.06%（78/229）and the multiple genotypes infection rate was 16.59%（38/229）.The total HPV positive rates,single and multiple genotype infection had statistically significantly differences between the two groups（P〈0.05）.Conclusion The HPV types 16,18,33,42,43,52,58 are the predominant genotypes in normal cervical cells and ASC-US. PCR combined with the gene-chip technology can be used in the HPV genotype detect in cervical cells,conduces to perform the fur-ther distribution management on ASC-US and has the important significance to prevention and control of cervical cancer.

关 键 词：宫颈肿瘤 癌 鳞状细胞 人乳头瘤病毒 基因型 

分 类 号：R737.33[医药卫生—肿瘤]