中国西部人群K-ras基因SNP位点多态性在胰腺癌中的表达及意义  

SNP polymorphism of K-ras of pancreatic cancer in China western people

作　　者：李汛[1] 芮少珍 严俊[1] 何雯婷[1] 周文策[1] 张磊[1] 孟文勃[1] 白仲添[1] 朱克祥[1] 朱晓亮[1] 

机构地区：[1]兰州大学第一医院普外二科,甘肃兰州730000

出　　处：《西部医学》2014年第7期822-824,共3页Medical Journal of West China

基　　金：甘肃省科技计划项目(0805TCYA040)

摘　　要：目的通过对胰腺癌患者手术切除肿瘤组织K-ras基因突变位点进行分析,探讨检测K-ras基因突变对胰腺癌的诊断预测价值。方法选取170例健康良性对照与66例病理证实胰腺癌患者,提取石蜡组织基因组DNA,使用MassARRAY时间飞行质谱技术进行K-ras基因突变分析。采用Fisher’s精确检验进行统计学分析。结果正常对照胰腺组织未检出阳性;66例胰腺癌组织中,8例K-ras基因第12位密码子(位点位于K-ras 12c1G)突变,通过Fisher’s精确检验统计分析,χ2值为21.329(P<0.001),差异具有显著统计学意义;本实验检测的其余两个位点K-ras12c2、K-ras 13未见突变。结论 K-ras基因第12位密码子(K-ras 12c1G)突变可能是胰腺癌变发生发展过程中的一个重要过程和信号。Objective We analyze K-ras gene mutation from pancreatic cancer and discusse the predictive value of K-ras gene mutation in diagnosis of pancreatic cancer.Methods 170 healthy controls and 66 patients with pancreatic cancer confirmed by pathology were involved in the present study.We extracted genomic DNA from paraffin-embedded tissues,used time of flight mass spectrometry（the MassARRAY technology）for K-ras gene mutation analysis.Results K-ras mutation of healthy controls was not detected.K-ras gene of 8 cases mutated at codon 12（site located K-ras12c1G）（χ2=21.329,P〈0.0010）.Conclusion K-ras gene at codon 12（K-ras 12c1G）mutation maybe is an important process and signals in development of pancreatic cancer.

关 键 词：胰腺癌 K-RAS基因 单核苷酸多态性 

分 类 号：R735.9[医药卫生—肿瘤]