外源性表达VEGF165b对人膀胱癌裸鼠移植瘤生长影响的研究  

Effects of Exogenous VEGF165b on Nude Mice Model of Bladder Cancer in Situ

作　　者：张金[1] 尹健[2] 张斯棋[3] 冯树强[1] 李然伟[1] 

机构地区：[1]吉林大学第二医院泌尿外科,吉林长春130041 [2]吉林大学中日联谊医院血管外科,吉林长春130033 [3]吉林大学中日联谊医院肾病内科,吉林长春130033

出　　处：《中国实验诊断学》2014年第7期1066-1068,共3页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省自然科学基金(200905139)

摘　　要：目的建立人膀胱癌T24细胞裸鼠移植瘤模型,探讨外源性表达VEGF165b对其生长的影响及其机制。方法分别移植未转染和转染VEGF165b表达载体的人膀胱癌T24细胞到裸鼠膀胱内,28d使处死动物,称量肿瘤重量,测定肿瘤体积。Western blot法检测肿瘤VEGF165b、p-VEGFR2、AKT、p-AKT蛋白表达。结果转染pcDNA-VEGF165b组肿瘤重量和体积与未转染组和转染pcDNA3.0组相比明显降低(P<0.05),而未转染组和转染pcDNA3.0组比较无统计学差异(P>0.05)。Western blot结果显示,转染pcDNA-VEGF165b组与未转染组和转染pcDNA3.0组比较,VEGF165b表达增高,p-VEGFR2、p-AKT表达降低,而未转染组和转染pcDNA3.0组间无明显差异。结论外源性表达VEGF165b可通过抑制VEGFR2信号通路传导而明显抑制人膀胱癌裸鼠移植瘤生长。Objective Establish the transplantation tumor model of human bladder cancer T24 cells in nude mice and explore the exogenous expression of VEGF165b on its growth and its mechanism.Methods Human bladder cancer T24 cells were transplant into bladders of nude mice.The mice were killed in 28d after transplantation.Western blot was used to confirm the protein expression of VEGF165b、p-VEGFR2、AKT、p-AKT.Results Compared with untreated group and pcDNA3.0 group,the weight and volume of tumor tissue were decreased in pcDNA-VEGF165b group（P〈0.05）.But there was no significant difference between untreated group and pcDNA3.0 group（P 〉0.05）.The protein expressions indicate that compared with untreated group and pcDNA3.0 group,VEGF165b was increased;p-VEG-FR2 and p-AKT were decreased.But there was no significant difference between untreated group and pcDNA3.0 group.Conclusion Exogenous expression of VEGF165b suppresses signaling pathway of VEGFR2 and inhibits the growth of nude mice model of bladder cancer.

关 键 词：膀胱癌 血管内皮生长因子165b 肿瘤侵袭 

分 类 号：R737.14[医药卫生—肿瘤]