贮藏温度和时间对滇杨花粉萌发的影响  

Effects of Storage Temperature and Time on Populus yunnanensis Pollen Germination

作　　者：赵雁鸣[1] 张雪娟[2] 赵月明[1] 李佳蔓[1] 李旦[3] 何承忠[1,2] 

机构地区：[1]西南山地森林资源保育与利用省部共建教育部重点实验室,西南林业大学,云南昆明650224 [2]西南地区生物多样性保育国家林业局重点实验室,西南林业大学,云南昆明650224 [3]云南生物多样性研究院,西南林业大学,云南昆明650224

出　　处：《贵州农业科学》2014年第6期133-136,共4页Guizhou Agricultural Sciences

基　　金：林业公益性行业科研专项项目"西南地区乡土杨树资源与利用研究"(201104076);国家自然科学基金项目"滇杨枝条倒插生根过程中生长素极性运输机制研究"(31360184);云南省中青年学术与技术带头人后备人才培养基金项目(2012HB021)

摘　　要：为探索滇杨花粉长期保存的适宜贮藏条件,采用离体培养法筛选滇杨花粉萌发适宜的蔗糖浓度,在适宜蔗糖浓度基础上,研究不同贮藏温度和时间滇杨花粉萌发率的变化。结果表明:滇杨花粉在1%琼脂+15g/L蔗糖+0.01%硼酸的培养基上萌发率最高,达34.84%;花粉萌发率因贮藏温度不同存在显著差异,在-80℃下贮藏240d,萌发率为28.35%,显著高于同时期内其他温度贮藏处理;花粉萌发率随贮藏时间的延长呈下降趋势,相同时间内贮藏温度越低花粉萌发率越高,室温下花粉萌发率下降最快。Effects of different sucrose concentrations on pollen germination rates of P .yunnanensis in vitroculture were investigated.According to the result,germination rates of pollen stored under different temperature and time were tested. It aimed to explore the suitable storage method for long-term preservation.The results showed that the highest pollen germination rates of P .yunnanensis was 34.84%on the medium composed of 1% ager,15 g/L sucrose and 0.01% boric acid.Significant differences were observed among pollen stored under different temperatures.After 240 days of storage at 80℃ pollen germination rate was 28.35%,which was significantly higher than pollen germination rates stored under the other three temperatures.It also showed that with the extension of storage time,pollen viability decreased,and at the same time the storage temperature lower,pollen viability was higher,the fastest decline in pollen viability at room temperature.

关 键 词：滇杨 花粉 离体萌发 贮藏温度 

分 类 号：S792.118[农业科学—林木遗传育种]