二价金属转运体1参与脉络丛上皮细胞铅吸收的机制  

The role of DMT1 in lead uptake in choroidal epithelial Z310 cells

作　　者：于海波[1] 张洁琼[1] 宋晗[1] 骆文静[1] 陈景元[1] 郑刚[1] 

机构地区：[1]第四军医大学劳动与环境卫生学教研室特殊作业环境危害评估与防治教育部重点实验室,陕西西安710032

出　　处：《实用预防医学》2014年第7期769-772,共4页Practical Preventive Medicine

基　　金：国家重点基础研究发展计划(973计划)课题(2012CB525002);国家自然科学基金项目(81230063)

摘　　要：目的研究二价金属转运体1(DMT1)参与脉络丛上皮细胞系Z310细胞铅转运的机制。方法采用原子吸收分光光度法检测Z310细胞铅吸收的时间效应。免疫荧光法观察DMT1在Z310细胞内的表达。采用siRNA干涉DMT1表达,荧光实时定量PCR检测DMT1的表达水平。原子吸收分光光度法检测DMT1 siRNA干涉对细胞铅吸收影响。结果醋酸铅暴露后,Z310细胞铅吸收量呈时间-依赖性增高;DMT1在Z310细胞中有较丰富的表达,主要在胞质中呈较均匀的分布。与阴性对照组相比,siRNA干涉后,Z310细胞DMT1 mRNA表达水平下降了74.8%。DMT1siRNA干涉后细胞铅吸收量减少了52.3%(P<0.01)。结论在脉络丛上皮细胞吸收铅的过程中,DMT1起到重要的转运作用。Objective To study the role of divalent metal transporter1 （DMT1） in lead uptake in choroidal epithelial Z310 cells. Methods Atomic absorption spectrophotometry was employed to determine the time- course of the cellular Pb uptake. The expression of DMT1 in Z310 cells was determined by immunofluorescence. Real- time RT- PCR was performed to test the effectiveness of DMT1 siRNA knockdown. The effect of DMT1 siRNA knockdown or L- type calcium channel inhibition on the cellular Pb uptake was determined by atomic absorption spectrophotometry. Results The intracellular Pb level increased time - dependently upon the addition of Ph. DMT1 expressed abundantly in Z310 cells and distributed mainly in cytoplasm. Real - time RT - PCR showed that DMT1 siRNA transfection resulted in a decreased DMT1 mRNA level by 74.8 %. The results from atomic absorption spectrophotometry showed that the Pb uptake was decreased by 53.8 % following the DMT1 siRNA knock- down. Conclusions DMT1 plays an important role in the cellular Pb uptake in choroidal epithelial Z310 cells.

关 键 词：铅 二价金属转运体1 脉络丛上皮细胞 铅吸收 DMT1 

分 类 号：R741.02[医药卫生—神经病学与精神病学]