化疗药物对细胞因子诱导的杀伤细胞(CIK)的影响  

Effect of Chemomedicine on Cytokine-Induced Killer( CIK) Cells

作　　者：焦雪[1] 王士勇[1] 刘畅[1] 刘迪杰[1] 单风平[2] 

机构地区：[1]中国医科大学附属第四医院生物治疗科,辽宁沈阳110032 [2]中国医科大学基础医学院免疫教研室,辽宁沈阳110001

出　　处：《微生物学杂志》2014年第3期42-46,共5页Journal of Microbiology

基　　金：国家自然科学基金(81200573);辽宁省自然科学基金(201102296)

摘　　要：探讨化疗药物及地塞米松对CIK细胞增殖活性及淋巴细胞亚群的影响。分别选取不同浓度顺铂、氟尿嘧啶、紫杉醇和地塞米松与不同诱导天数的CIK细胞共培养,在加入或不加入IL-2的情况下,采用MTT法检测各种药物对CIK细胞增殖活性的影响,流式细胞术检测CIK细胞中各亚群的比例。顺铂、氟尿嘧啶、紫杉醇及地塞米松均在一定程度上抑制CIK细胞的增殖活性,其中顺铂和紫杉醇表现出明显的剂量依赖性(P<0.05);IL-2可对CIK细胞产生保护作用,在一定程度上降低化疗药物对CIK细胞的抑制作用(P<0.05)。顺铂主要降低CD3+CD56+亚群比例(P<0.05),对CD3+CD4+、CD3+CD8+亚群比例无显著影响(P>0.05)。Effects on proliferation viability and proportions of lymphocyte subsets by chemomedicine and dexamethasone were investigated. By means of MTT, the proliferation viability of CIK cells that selected different concentration of CDDP, 5-FU, paclitaxel and dexamethasone respectively to coculture with different days’CIK cells with or without IL-2 was tested; flow cytometry was used to measure the proportions of lymphocyte subsets. CDDP, 5-FU, paclitaxel and dexamethasone all could inhibit the proliferative viability of CIK cells to some extent, especially, CDDP and paclitaxel both had dose dependent action significantly （P〈0.05）. IL-2 helped to protect the CIK cells, which decreased the inhibition of chemomedicine affected on CIK cells （P〈0.05）. Effect of CDDP to lymphocyte subsets mainly was that the proportions of CD3^＋CD56^＋ subsets decreased （P〈0.05）, while to CD3^＋CD4^＋ and CD3^＋CD8^＋ subsets had no significant effect （P〉0.05）.

关 键 词：化疗药物 细胞因子诱导的杀伤细胞 MTT 淋巴细胞亚群 

分 类 号：Q939.93[生物学—微生物学] R730.5[医药卫生—肿瘤]