ILK和TGF-β1在实验性大鼠肝纤维化中的表达和意义  

Expression and significance of ILK and TGF-β1 in experimental hepatic fibrosis

作　　者：毛忠懿[1] 吴雄健[1] 陶立刚[1] 

机构地区：[1]江西省赣南医学院第一附属医院,江西赣州341000

出　　处：《安徽医药》2014年第9期1630-1633,共4页Anhui Medical and Pharmaceutical Journal

基　　金：江西省卫生厅科技计划项目(No 20133091)

摘　　要：目的研究转化生长因子β1(TGF-β1)和整合素连接激酶(ILK)在大鼠肝纤维化中的表达和意义。方法采用40%CCl4诱导的大鼠肝纤维化模型。60只SD大鼠随机分为五组:正常对照组,模型2周组,模型4周组,模型6周组,模型8周组。对2、4、6、8周肝纤维化模型及对照组大鼠HE染色、网状纤维染色、免疫组化反应,观察肝组织病理变化、纤维增生及ILK和TGF-β1的表达情况。结果随着肝纤维化的发展,ILK和TGF-β1在2、4、6、8周组中表达程度呈明显递增趋势,表明大鼠肝组织纤维化与ILK和TGF-β1的表达相关。结论 ILK和TGF-β1的表达可能和大鼠肝纤维化有联系,或许可为肝纤维化的防治提供新的理论依据。Objective To study the expression and significance of transforming growth factor β1 （ TGF-β1） and integrin-linked kinase （ ILK） in experimental hepatic fibrosis in rats .Methods Liver fibrosis models of rats were induced by injecting 40% CCl4 .Sixty SD rats were randomized into five groups：normal control group ,2-week model group ,4-week model group ,6-week model group ,8-week model group.2-,4-,6-,8-week liver fibrosis model and control group rats were stained by HE staining ,reticular fiber staining,and im-munohistochemical reactions ,and then the pathological change ,fibrous proliferation and expression of TGF-β1 and ILK in rat liver tis-sues were observed .Results With the development of liver fibrosis ,expressions of ILK and TGF-β1 significantly increased in 2-,4-, 6-,8-week model groups ,which indicated that liver fibrosis in rats was correlated with the expressions of ILK and TGF-β1.Conclusions The expressions of ILK and TGF-β1 may be associated with liver fibrosis in rats ,which can provide a new theoretical basis for the prevention and treatment of liver fibrosis .

关 键 词：转化生长因子Β1 整合素连接激酶 肝纤维化 

分 类 号：R575[医药卫生—消化系统]