原发性胆汁性肝硬化患者血清细胞因子表达及意义  

Expression and significance of serum cytokine profiles in patients with primary biliary cirrhosis

作　　者：梁文学[1] 杨新玲[1] 杨晋[1] 赵绍林[1] 吴惠毅[1] 

机构地区：[1]连云港市第一人民医院中心实验室,江苏省222002

出　　处：《中华临床医师杂志（电子版）》2014年第13期36-39,共4页Chinese Journal of Clinicians(Electronic Edition)

摘　　要：目的探讨原发性胆汁性肝硬化(PBC)患者血清中16种相关炎性因子表达及其临床意义。方法以50例PBC患者和40名健康对照者作为研究对象,应用Luminex液态芯片系统检测16种炎性因子血清表达情况。分析表达存在明显差异细胞因子与PBC临床及实验室参数相关性。指标相关性采用Spearman相关性检验,数值资料比较采用t检验。结果与健康对照组相比,PBC组血清中IL-6、IL-8、IL-17A、IFN-γ和TNF-α表达显著升高,差异有统计学意义[(5.8±1.3)pg/ml,(69.7±22.1)pg/ml;(328.5±154.5)pg/ml,(874.5±678.5)pg/ml;(21.3±8.5)pg/ml,(68.5±32.5)pg/ml;(25.6±10.5)pg/ml,(104.5±46.8)pg/ml;(125.5±69.5)pg/ml,(408.5±185.6)pg/ml;P均<0.01]。IL-6、IL-17A、IFN-γ和TNF-α血清表达与PBC Mayo风险评分呈正相关,相关系数r分别为0.598、0.618、0.519和0.614,P均<0.05。IL-17A、IFN-γ和TNF-α血清表达与ALT、ALP及CRP水平呈正相关,相关系数r分别为:0.632、0.521、0.493;0.614、0.603、0.532;0.727、0.814、0.659;P均<0.05。结论 PBC患者血清炎性因子IL-6、IL-17A、IFN-γ和TNF-α表达显著升高,且与疾病活动指数呈相关。ObjectiveTo investigate the clinical significance of 16 related serum inflammatory factors expression in patients with primary biliary cirrhosis （PBC）.Methods The serum levels of the 16 inflammatory factors from 50 patients of PBC and 40 healthy controls were detected by the liquid chip system. The correlations of the inflammatory factors, which had a significant difference between the PBC and controls, was further analyzed. Spearman correlation test was used in index correlation using, and t-test was used to compare the numerical data.Results Comparing with healthy-control group, expression of serum cytokines such as IL-6, IL-8, IL-17, IFN-γ and TNF-α were significantly higher in PBC（P〈0.01）. The expression of IL-6, IL-8, IL-17, IFN-γ and TNF-α were positively related to the Mayo risk score （r were 0.598, 0.618, 0.519 and 0.614 respectively（P〈0.05）. The expression of IL-17, IFN-γ and TNF-α were positively related to the ALT, ALP and CRP （r were 0.632, 0.521, 0.493, 0.614, 0.603, 0.532, 0.727, 0.814 and 0.659 respectively,P〈0.05）.Conclusion The serum level of the cytokines such as IL-6, IL-8, IL-17, IFN-γ and TNF-α were significantly increased in PBC and positively related to the disease activity index.

关 键 词：肝硬化 胆汁性 自身免疫疾病 细胞因子类 

分 类 号：R575.2[医药卫生—消化系统]