多发性骨髓瘤中p15基因甲基化及其mRNA表达的研究  

Study on methylation and mRNA expression of p15 gene in multiple myeloma

作　　者：李灿美 杨凌[2] 刘琳[3] 李一辉 杨善兰 金美四 贺振新[2] 

机构地区：[1]大理州人民医院血液肿瘤科,云南大理671000 [2]昆明医科大学第一附属医院血液科,云南昆明650032 [3]昆明医科大学第二附属医院血液科,云南昆明650101

出　　处：《云南医药》2014年第3期274-278,共5页Medicine and Pharmacy of Yunnan

基　　金：云南省应用基础研究面上项目:2009CD172

摘　　要：目的探讨p15基因的甲基化及其mRNA转录阻抑与多发性骨髓瘤(MM)的发病和进展之间的关系。方法采用甲基化特异性聚合酶链反应(MSP)检测54例初治和复发MM患者及40例正常骨髓的p15基因启动子区的甲基化状态,并对MSP产物进行序列测定;采用荧光实时定量PCR(RT-qPCR)方法检测MM患者及对照组p15基因mRNA表达情况。结果p15基因启动子区甲基化阳性率为27.78%(15/54),与对照组相比,差异有统计学意义(P<0.05)。p15基因甲基化率与患者的性别、年龄、病程阶段差异无统计学意义(P>0.05)。MM组与正常对照组,甲基化组与非甲基化组,初诊组与复发组相比较p15基因mRNA表达均无显著降低,差异无统计学意义(P>0.05)。结论p15基因的甲基化与MM的发病有关,与患者的性别、年龄、病程阶段无关。p15基因甲基化与其mRNA转录阻抑的相关性不高。Objective To illustrate the role of methylation of the p15 genes and their mRNA expression in the pathogenesis of multiple myeloma（MM）.Methods The methylation of the p15 gene promoter region in bone marrow from 54 cases of newly diagnosed or relapsed MM and 40 of normal subjects were analyzed by using methylation-specific PCR methods （MSP）, and the product of MSP was sequenced; the methylation status in the p15 genes and their mRNA expression were analysed. Results The methylation rates for the p15 gene promoter region were 27.78% （15/54） in MM, and there were a significant differences between MM patients and the control group（P 〈 0.05）.However, there were no differences of the p15 gene methylation rates among the clinico-pathological data such as sex, age, and duration of stage （P 〉 0.05）. The p15 gene mRNA expression between MM group and the normal control group, the methylation group between the non-methylation of group newly diagnosed or relapsed diagnosed were not significantly reduced, the differences were not statistically significant （P 〉 0.05）.Conclusion The results suggest that methylation of p15 genes plays an important role in MM tumouregenesis,however,there are no differences of the 15 gene methylation rate among the clinico-pathological data such as sex, age and duration of stage. There is not a strong correlation between methylation and transcriptional repression of p15 genes.

关 键 词：多发性骨髓瘤 P15基因 甲基化 

分 类 号：R733.3[医药卫生—肿瘤]