5-异丁基-3-(2-氯)苄氧基乙内酰脲对白血病细胞K562生长与凋亡的影响  

Effect of 3-(2-Chlorobenzyloxy)-5-Isobutylhydantoin on Growth and Apoptosis of Leukemia K562 Cells

作　　者：仲英[1,2] 林莉[1] 麦曦[1] 冯丽华[1] 廖一静[1] 魏金金[1] 

机构地区：[1]南昌大学药学院药物分析教研室,南昌330006 [2]江西省药品审评中心,南昌330046

出　　处：《南昌大学学报（医学版）》2014年第5期11-14,26,共5页Journal of Nanchang University：Medical Sciences

基　　金："重大新药创制"国家科技重大专项项目(2009ZX09103-087);江西省教育厅科技项目(12027)

摘　　要：目的：探讨5-异丁基-3-（2-氯）苄氧基乙内酰脲（YL3）对白血病细胞 K562细胞生长与凋亡的作用。方法将K562细胞置于含10％FBS的 RPMI-1640培养液中培养。实验分4个组：空白对照组、阴性对照组、阳性对照组（HU组）、受试药物组（YL3组）；除空白对照组外，其余3组均接种K562细胞；HU组、YL3组分5个亚组，分别加入10-3～10-7 mol·L-15个梯度浓度的 HU或 YL3。采用 MTT法检测 YL3对 K562细胞增殖的影响；AV/PI双染流式细胞术检测 YL3对 K562细胞凋亡的影响；瑞氏染色法观察细胞形态的变化。结果 YL3在10-3～10-6 mol·L-1浓度范围内显著抑制 K562细胞增殖（P＜0．01或 P＜0．05），其半数细胞抑制浓度（IC50）值为88．5μmol·L-1；YL3有促进K562细胞凋亡的作用，其高剂量的凋亡率显著高于同剂量的 HU组（P＜0．01），且呈量效关系（P＜0．05）。结论5-异丁基-3-（2-氯）苄氧基乙内酰脲具有抑制 K562细胞增殖，诱导其凋亡的作用。Objective To explore the effect of 3-（2-chlorobenzyloxy ）-5-isobutylhydantoin （YL3）on the growth and apoptosis of leukemia K562 cells.Methods Leukemia K562 cells were cultured in RPMI-1640 supplemented with 10% fetal bovine serum.Cells were divided into four groups：blank control group,negative control group,positive control group（HU group）and YL3 group.Furthermore,cells in HU group and YL3 group were treated with HU and YL3 at concen-trations of 10-3-10-7 mol·L-1,respectively.The proliferation and apoptosis were detected by MTT assay and A V/PI flow cytometry,respectively.Changes in cellular morphology were ob-served by Wight-Giemsa staining.Results YL3 treatment（10-3-10-6 mol·L-1 ）significantly in-hibited the growth of leukemia K562 cells（P〈0.01 or P〈0.05）.The half-maximal inhibitory concentration（IC50）was 88.5μmol·L-1.In addition,YL3 promoted the apoptosis of leukemia K562 cells in a dose-dependent manner（P〈0.05）,and the apoptosis rate in high-dose YL3 group was significantly higher than that in high-dose HU group（P〈0.01）.Conclusion YL3 treatment can inhibit the growth and induce the apoptosis in leukemia K562 cells.

关 键 词：5-异丁基-3-(2-氯)苄氧基乙内酰脲 K562 细胞 MTT法 细胞生长 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤]