无溶剂体系脂肪酶动力学拆分2-辛醇及产物的非均相共沸蒸馏提取  

Kinetics resolution of 2-octanol by lipase in solvent-free system and separation of the product via azeotropic distillation

作　　者：任立伟[1] 徐田[1] 蒋振华[1] 贾红华[1] 周华[1] 韦萍[1] 

机构地区：[1]南京工业大学生物与制药工程学院,南京211800

出　　处：《生物加工过程》2014年第4期55-59,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家重点基础研究发展计划(973计划)(2009CB724706)

摘　　要：为了提高脂肪酶在非水相中对手性仲醇的拆分效率,以2-辛醇为模式底物,建立了以辛酸为酰基供体的无溶剂脂肪酶动力学拆分手性仲醇的反应体系,采用1.5∶1的酸醇摩尔比,45℃条件下反应12 h,2-辛醇的转化率达到49.9%,并且S-2-辛醇的对映体过量率e.e.s=98.2%,反应的对映体比率E>600。利用脂肪族仲醇与水形成共沸物的特性,通过非均相共沸蒸馏的方法提取拆分得到的手性仲醇,S-2-辛醇的光学纯度并未降低,并且产率大于90%,产品纯度大于98%。In order to enhance the efficiency of the kinetics resolution of secondary alcohols by lipase in non-aqueous phase, a solvent-free system was established using 2-octanol and octanoic acid as the model substrate and the acyl donor, respectively. When the reaction was carried out with n（ acid）∶n（ alcohol）=1.5∶1 at 45 ℃ for 12 h, the （ S）-2-octanol was obtained in e. e.=98.2% and the enantiomeric ratio reached E＆gt;600. The ability of aliphatic secondary alcohols to form an azeotrope with water was exploited to distill the enantiomerically pure secondary alcohol from the solvent-free system. The ee value of S-2-octanol was perfectly maintained. Furthermore, the yield and the purity of （ S）-2-octanol were more than 90% and 98%, respectively.

关 键 词：共沸蒸馏 动力学拆分 脂肪酶 手性仲醇 

分 类 号：TQ033[化学工程]