HPPH光动力学治疗大鼠C6脑胶质瘤实验研究的磁共振波谱技术MRS的变化  

Changes of Magnetic Resonance Spectroscopyin the Photodynamic Therapy with 2-[1-Hexyloxyethyl]-2-Devinyl Pyropheophorbide-alpha(HPPH)on C6 Glioma in the Rat

作　　者：王宇[1] 沈加林[2] 林勇[1] 张慧国[3] 赵子周[2] 刘桂勤[2] 王磊[1] 朱菁[3] 

机构地区：[1]上海交通大学医学院附属仁济医院神经外科,上海200127 [2]上海交通大学医学院附属仁济医院放射科,上海200127 [3]上海交通大学医学院附属仁济医院皮肤科,上海200127

出　　处：《应用激光》2014年第3期247-257,共11页Applied Laser

基　　金：技术委员会动物专项资助项目(项目编号:11140900360)

摘　　要：目的：是研究不同剂量新型叶绿素光敏剂HPPH光动力学疗法治疗大鼠C6脑胶质细胞移植瘤模型,以磁共振波谱技术（MRS）无创观察不同时间的变化、评价疗效及选择光敏剂HPPH最佳剂量并与常规光敏剂血卟啉衍化物（HPD）光动力学治疗及对照组作对比。方法：建立大鼠C6脑胶质细胞瘤模型,设立对照组（空白对照组、单注射HPPH0.45mg/kg组、单注射HPD5mg/kg组、单波长667nm激光照射组、单波长630nm激光照射组）、HPPH-PDT各组（HPPH0.15、0.30、0.45mg/kg组）、HPD-PDT5mg/kg组。单注射HPPH组、HPPH-PDT组和单注HPD组、HPD-PDT组自尾静脉推注入光敏剂,光动力学治疗组注光敏剂后18小时进行激光照射.HPPH-PDT各组和单667nm激光照射组肿瘤以波长667nm的半导体激光照射,功率密度200mW/cm2,每光斑照射20min,能量密度为240J/cm2;HPD-PDT组及单630nm激光照射组肿瘤以波长630nm的半导体激光照射,功率密度200mW/cm2,每光斑照射20min,能量密度为240J/cm2。以磁共振平扫、增强扫描及磁共振波谱技术（MRS）观察光动力学治疗组及对照组肿瘤不同时间的变化,光动力学治疗前、治疗后7、14天（P0、P7、P14天水）;接种后第7、14、21天;（T7、T14、T21天）。于PDT后14天或肿瘤生长第21天磁共振扫描后处死鼠,取脑组织作病理检查。结果：本文观察大鼠C6脑胶质瘤模型接种不同时间段波谱cho/NAA均值,正常脑cho/NAA均值在第1、7、14、21天测定在0.5左右,而接种后的大鼠C6脑胶质瘤及未光动力学治疗各组7、14、21天测定波谱cho/NAA均值为5.532±4.066-5.574±3.382、6.488±2.169-6.744±3.685、8.684±5.42-8.938±4.08,随着肿瘤的长大该均值在不断加大。正常脑与移植瘤未光动力学治疗各对照组波谱cho/NAA值T-检验,P值小于0.05或0.001有显著或非常显著差异。而移植瘤未光动力学治疗各对照组、各时间组间P〉0.05,各组间无显著差异。光动力学治疗组chObjective：The aim is to use magnetic resonance spectroscopy（MRS）to investigate the efficacy of the photodynamic therapy（PDT）with different doses of the photosensitizer 2-[1-hexyloxyethyl]-2-devinyl pyropheophorbide-alpha（HPPH）on the C6 glioma in the rat,in which the optimal dose of the HPPH will be selected and the efficacy will be compared with that of the PDT with the hematoporphyrin derivative（HPD）.Methods：After setting up the C6 glioma model in the rat,the animals were divided into control groups（blank group,HPPH only（0.45mg/kg）group,HPD only（5mg/kg）group,667nm laser light only group,and 630nm laser light only group）,HPPH-PDT groups（HPPH0.15,0.30,and 0.45mg/kg groups）,and HPD-PDT（5mg/kg））group.The photosensitizers were injected into the rats via the tail veins.18h after the injection of the photosensitizers,while 667nm laser beam was used to irradiate the tumors in the groups of HPPH-PDT and the group of 667nm laser light only,the tumors of the HPD-PDT group and 630nm laser light only group were treated with 630nm laser beam at a laser intensity of 200mW/cm2 and an energy density of 240J/cm2 for 20min per photo spot.The magnetic resonance imaging（MRI）and MRS were used to observe the tumor changes in the PDT groups and control groups before and 7and 14days after the PDT（P0,P7,P14）,that is,7,14,and 21 days after tumor implantation（T7,T14,T21）.The rats were sacrificed for pathological examination 14days after the PDT or on 21st day after tumor implantation.Results：The choline/n-acetylaspartate（Cho/NAA）value of the MRS was measured.It was approximately 0.5in the normal rat brain,but the values were significantly increased to 5.532±4.066-5.574±3.382,6.488±2.169-6.744±3.685,and 8.684±5.42-8.938±4.08on day 7,14,and 21after glioma cell implantation,respectively（P〈0.05or〈 0.01）,which was elevated with the tumor growth.There are no significant difference among the tumor groups（P〉0.05）.After the PDT,the Cho/NAA values of 0.832±0.334,0.818±

关 键 词：大鼠C6脑胶质细胞瘤 PPH光动力学治疗 磁共振MRS 

分 类 号：R739.41[医药卫生—肿瘤]