RhoA对食管癌Eca-109细胞VEGF表达的调控作用  

The regulatory mechanism of RhoA on VEGF expression in esophageal squamous cell carcinoma cell line Eca-109

作　　者：马骥[1] 赵庆丽[1] 钟翠萍[1] 刘文超[2] 薛妍[2] 

机构地区：[1]兰州军区兰州总医院乳腺科,兰州730050 [2]第四军医大学西京医院肿瘤中心,710032

出　　处：《临床肿瘤学杂志》2014年第6期486-489,共4页Chinese Clinical Oncology

基　　金：国家自然科学基金资助项目(81202085;81201209;81372478)

摘　　要：目的探讨小G蛋白超家族成员RhoA对食管癌Eca-109细胞血管内皮生长因子(VEGF)表达的调控作用。方法食管癌Eca-109细胞转染过表达RhoA质粒(V14RhoA)或沉默表达RhoA质粒(shRhoA)后,采用Western blotting和酶联免疫吸附试验(ELISA)法分别检测其细胞内外VEGF蛋白表达,Western blotting和Real-time PCR分别检测Eca-109细胞转染V14RhoA和shRhoA后p53和VEGF表达的情况。结果 Western blotting和ELISA法检测显示,Eca-109细胞转染V14RhoA后细胞内、外VEGF表达水平升高,而转染shRhoA后VEGF的表达被显著抑制。Western blotting和Real-time PCR检测显示,Eca-109细胞转染V14RhoA后p53表达显著降低,而VEGF表达显著升高(P<0.05)。结论食管癌Eca-109细胞中RhoA表达的变化可以引起细胞内外VEGF水平的变化,并且RhoA可通过抑制p53表达从而增加对VEGF的调控。Objective To explore the effect and mechanism of RhoA on vascular endothelial growth factor（ VEGF） expression and regulation in esophageal carcinoma cell line Eca-109. Methods Eca-109 cells were transfected by RhoA up-regulating plasmid（ V14RhoA） or down-regulating plasmid（ shRhoA） and VEGF expression level was examined by Western blotting and ELISA assay.The effect of RhoA on p53 expression was examined by Western blotting and real-time PCR. Results Western blotting and ELISA assays showed that in Eca-109 cells VEGF protein expression was increased or decreased with up-regulating or down-regulating RhoA.Western blotting and real-time PCR assays showed that increased RhoA could inhibit p53 expression and then promote VEGF expression. Conclusion In Eca-109 cells,RhoA can regulate VEGF expression,and RhoA promotes VEGF expression through inhibiting p53 expression.

关 键 词：RHOA 食管癌 血管内皮生长因子 P53 

分 类 号：R735.1[医药卫生—肿瘤]