3’3-二吲哚甲烷联合5-氟尿嘧啶对胃癌细胞增殖的影响  

The inhibitory effect of 3,3'-Diindolylmethane plus 5-fluorouracil on gastric cancer cell proliferation

作　　者：苏明丽[1] 卢文华[2] 陈洁[1] 

机构地区：[1]中山大学附属第一医院消化内科,广东广州510080 [2]中山大学肿瘤防治中心 华南肿瘤学国家重点实验室,广东广州510080

出　　处：《热带医学杂志》2014年第6期697-700,共4页Journal of Tropical Medicine

基　　金：国家自然科学基金(30871145;81072048);教育部高等学校博士学科点专项科研基金(20100171110062);中山大学重大项目培育和新兴;交叉学科资助计划(10ykjc23);高校基本科研业务费中山大学青年教师培育项目(09ykpy22)

摘　　要：目的探讨3’3-二吲哚甲烷（DIM）联合化疗药物5-氟尿嘧啶（5-FU）对胃癌细胞增殖的影响。方法 MTT法检测DIM、5-FU单药及联用对不同分化程度胃癌细胞株（MKN28、SGC7901、AGS、MKN45、HGC27）增殖的影响,计算药物对不同胃癌细胞的半数抑制浓度IC50及联用效应。结果 DIM及5-FU单药对不同分化程度胃癌细胞增殖的半数抑制浓度（IC50）范围分别为23~42μmol/L及9.5~95.9μmol/L,两者联用对胃癌细胞的抑制率较单药高,但仅对中分化胃癌细胞株SGC7901和低分化胃癌细胞株MKN45的联用指数（CI）均介于0和1之间。结论 DIM及5-FU单药均能抑制体外培养的不同分化程度胃癌细胞增殖,两者联用对抑制中分化胃癌细胞株SGC7901和低分化胃癌细胞株MKN45的增殖具有协同效应。Objective To observe the in vitro inhibitory effects of 3,3＇-Diindolylmethane（DIM） plus 5-fluorouracil（5-FU） on various gastric cancer cell lines（MKN28, SGC7901, AGS, MKN45, HGC27）. Methods The inhibitory effects of DIM and 5-FU either in combination or alone were measured by MTT assay and the synergistic effect of the two drugs was evaluated by calcusyn software according to the median-effect principle. Results The IC50 of DIM and 5-FU on all the gastric cancer cell lines were ranged from 23 to 42 μmol / L and 9.5 to 95.9 μmol / L, respectively. The combination of DIM and 5-FU showed an accelerated inhibitory effect,but the two agents were synergistic only when used concurrently on gastric cancer cell line SGC7901 and MKN45. Conclusions These results indicated that both DIM and 5-FU showed dose-dependent inhibitory effect on gastric cancer cell lines（MKN28, SGC7901, AGS, MKN45, HGC27）, and the combination of them was synergistic on gastric cancer cell line SGC7901 and MKN45.

关 键 词：胃癌 3 3-二吲哚甲烷 5-氟尿嘧啶 协同作用 

分 类 号：R735.2[医药卫生—肿瘤]