实验性自身免疫性葡萄膜炎大鼠的发病特点  

The inflammatory characteristics of experimental autoimmune uveitis

作　　者：刘正峰[1] 崔彦[2] 李姣[2] 滕达[1] 唐凯[1] 毕宏生[2] 

机构地区：[1]山东中医药大学,济南250002 [2]山东中医药大学附属眼科医院 山东中医药大学眼科研究所,济南250002

出　　处：《中华实验眼科杂志》2014年第7期588-592,共5页Chinese Journal Of Experimental Ophthalmology

基　　金：国家自然科学基金项目（81072961、81100658）;山东省自然科学基金项目（ZR2010HM048）;山东中医药科技发展计划项目（2013ZDZK-083）;山东省医药卫生科技发展计划项目（2013WS0251）

摘　　要：背景 Lewis大鼠是建立实验性自身免疫性葡萄膜炎（EAU）动物模型的常用种系,对其发病特点,尤其是EAU大鼠眼部超微结构改变的研究尚未见报道. 目的 观察EAU大鼠的发病体征及其眼部超微结构的改变. 方法 选取SPF级6～8周龄雌性Lewis大鼠18只,采用随机数字表法随机分为对照组6只和模型组12只.模型组大鼠后肢足底、两侧腹壁和躯干上皮下各注射含有光感受器间维生素A类结合蛋白（IRBP,1177-1191）和结核菌素（TB）的完全弗氏佐剂（CFA）乳化液,对照组大鼠不作处理.注射后观察两组大鼠饮食、体温和活动情况,每天裂隙灯显微镜下观察大鼠眼部炎症表现,于免疫后12d获取大鼠眼组织标本,对虹膜、睫状体和视网膜进行常规组织病理学检查,并分别在扫描电子显微镜和透射电子显微镜下观察虹膜、睫状体和视网膜的超微结构改变.结果 模型组大鼠免疫后采食量为（190.00± 18.03）g,明显少于对照组的（285.33±28.02）g,差异有统计学意义（t=4.955,P=0.012）;模型组和对照组大鼠的饮水量分别为（241.67±18.56）ml和（289.67±18.18）ml,差异有统计学意义（t=3.201,P=0.033）;模型组大鼠体温升高,精神倦怠.裂隙灯显微镜下观察发现,模型组大鼠免疫后6d出现虹膜充血、前房积脓和瞳孔膜闭,免疫后12d眼部炎症最严重,炎症评分为（3.83±0.41）分,而对照组大鼠眼前节未见异常.组织病理学检查发现,模型组大鼠前房、虹膜、睫状体组织和玻璃体腔内均可见大量淋巴细胞、中性粒细胞和单核巨噬细胞浸润.扫描电子显微镜下可见模型组大鼠虹膜肌纤维粗细不均,睫状体上皮表面粗糙及RPE细胞绒毛疏松.透射电子显微镜下观察可见,模型组大鼠虹膜单核巨噬细胞浸润,睫状体上皮细胞膜褶皱蓬松及排列紊乱,视网膜Müller细胞中有髓样小体,RPE细胞中有线粒体空泡出现,而对照组大鼠虹膜、睫状体及Background Lewis rat is a commonly used specie in experimental autoimmune uveitis（EAU）.However,the characteristics of EAU,especially ocular uhrastructural change,are rarely reported.Objective This study was to investigate the inflammatory characteristics and ocular uhrastructure of EAU models in Lewis rat.Methods EAU models were induced in 12 SPF female Lewis rats（6-8 weeks old）by injection of complete Freund adjuvant（CFA） containing interphotoreceptor retinoid-binding protein （IRBP,1177-1191） and tuberculin （TB） into footpads,napes and back of the body,and 6 normal matched rats were used as normal controls.The diet and drinking,temperature and behavior acts of the rats were recorded during the observational duration.Ocular manifestations were examined under the slit lamp biomicroscope after modeling and scored.Eyeballs were obtained in 12 days after modeling for histopathological examination,and the ultrastructures of eyeballs were observed under the scanning electron microscope and transmission electron microscope.The use and care of the animals complied with Statement of ARVO.Results The food-intake was （190.00± 18.03）g in the model group,and that in the control group was （285.33 ±28.02） g,showing a significant difference between them （t =4.955,P =0.012）.The drinking-water volume of rats was （241.67±t 18.56）ml in the model group,which was significantly less than （289.67± 18.18）ml in the control group,showing a significant difference between them （t =3.201,P =0.033）.In addition,elevated temperature and tiredness were found in the model rats.Anterior chamber empyema,iris hyperemia and occlusion of the pupil appeared in the models on the 6th day and peaked on the 12nd day after immunized,with the inflammatory scores of 3.83±0.41.The infiltration of inflammatory cells were seen in anterior chamber,ciliary body and vitreous cavity under the optical microscope in the model rats.Scanning electron microscopy found uneven iris texture,coarse ciliary surface and loosen vi

关 键 词：实验性自身免疫性葡萄膜炎 炎症 超微结构 

分 类 号：R773.9[医药卫生—眼科]