AKT-Foxo3a信号通路调控小鼠MMP-20基因表达的研究  

AKT-Foxo3a signaling pathway in regulation of MMP-20gene expression in mouse ameloblasts

作　　者：梁广智[1,2] 王云虹[1] 刘媛媛[1] 慈浩粟[3] 荣丽[3] 韩晓谦[3] 孙轶群[3] 高玉光[3] 

机构地区：[1]潍坊医学院口腔学院,山东潍坊261053 [2]潍坊医学院附属医院口腔科,山东潍坊261031 [3]滨州医学院附属医院口腔科,山东滨州256603

出　　处：《牙体牙髓牙周病学杂志》2014年第6期323-326,337,共5页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助项目(81170927);潍坊医学院青年基金(k11qc101)

摘　　要：目的:本研究旨在探讨AKT-Foxo3a信号通路在基质金属蛋白酶-20(matrix metalloproteinase-20,MMP-20)基因表达中的作用。方法:分别通过免疫组化技术、实时定量RT-PCR技术以及双荧光素酶报告基因检测系统分析AKT-Foxo3a信号通路对MMP-20启动子转录活性的影响。结果:免疫组化染色结果显示,AKT及磷酸化Foxo3a(P-Foxo3a)均在分泌期成釉细胞核中呈阳性表达;RT-PCR检测结果显示,AKT阻断剂可显著抑制Foxo3a诱导的MMP-20基因的表达(P﹤0.05);双荧光素酶分析结果显示,AKT阻断剂亦可显著抑制Foxo3a调控MMP-20基因启动子的转录活性(P﹤0.05)。结论:转录因子Foxo3a可能通过AKT-Foxo3a信号通路调控MMP-20基因的表达,从而参与釉质发育过程。AIM： To investigate the role of AKT-Foxo3a signaling pathway in matrix metalloproteinase-20（ MMP-20） gene expression in mouse ameloblasts. METHODS： AKT-Foxo3a and MMP-20 expression in mouse ameloblasts were examined by immunohistochemistry. Effects of AKT-Foxo3a signaling pathway on MMP-20 promoter were investigated by real-time quantitative RT-PCR and dual luciferase reporter gene assay system. RESULTS： Immunohistochemical staining results showed that AKT and phosphorylated Foxo3a（ P-Foxo3a） were both strongly expressed in the nuclei of secretory ameloblasts. By RT-PCR,it was found that AKT inhibitor reduced the Foxo3a induced MMP-20 gene expression（ P〈0. 05）. Dual luciferase assay showed that AKT inhibitor decreased MMP-20 promoter activity（ P〈0. 05）. CONCLUSION： Foxo3a regulates MMP-20 gene expression through AKT-Foxo3a signaling pathway in mouse ameloblasts.

关 键 词：AKT FOXO3A MMP-20 

分 类 号：R780.2[医药卫生—口腔医学]