非霍奇金淋巴瘤患者外周血单核细胞hepcidin可能导致慢性病贫血  

Peripheral blood monocyte hepcidin from patients of non-Hodgkin's lymphoma may lead to anemia of chronic disease

作　　者：韩潇[1] 周道斌[1] 段明辉[1] 汪玄[1] 赵永强[1] 沈悌[1] 武永吉[1] 

机构地区：[1]中国医学科学院 北京协和医学院 北京协和医院 血液内科,北京100730

出　　处：《基础医学与临床》2014年第7期914-920,共7页Basic and Clinical Medicine

基　　金：北京市自然科学基金(7122140)

摘　　要：目的探讨非霍奇金淋巴瘤患者外周血单核细胞hepcidin可能发挥的作用。方法收集符合条件的非霍奇金淋巴瘤患者的临床资料及外周静脉血,ELISA法测定血清IL-6和TNF-α浓度,CD14+磁珠分选外周血单核细胞,实时定量PCR测定外周血单核细胞hepcidin,IL-6和TNF-αmRNA。结果非霍奇金淋巴瘤患者血红蛋白浓度低于正常对照(113±21.7)g/L,其外周血单核细胞hepcidin表达高于正常(P<0.05)。初治患者单核细胞hepcidin表达与血红蛋白浓度和血清IL-6负相关(P<0.05),但和TSAT%,SF及血清TNF-α无关联。结论初治淋巴瘤患者外周血单核细胞升高的hepcidin可能导致慢性病贫血的发生。Objective To explore the role of monocytes hepcidin in NHL patients.Methods Collecting the clinical information and peripheral venous blood of non-Hodgkin＇s lymphoma patients.Serum concentration of IL-6 and TNF-α was detected by ELISA.Peripheral blood monocytes were isolated by CD14 ＋ Magnetic beads.Hepcidin,IL-6 and TNF-α mRNA of monocytes were detected by Real time PCR quantitative assay.Results In NHL patients,hemoglobin was reduced （112.5 ± 21.7）g/L while monocyte hepcidin expression was higher than normal （P 〈 0.05）.Monocyte hepcidin in untreated patients was negatively correlated with Hb and IL-6 levels（P 〈 0.05）,but not with TSAT%,SF and TNF-α level.Conclusions The increased monocyte hepcidin in untreated lymphoma patients may play an etiologic role in ACD.

关 键 词：非霍奇金淋巴瘤 慢性病贫血 HEPCIDIN 白介素-6 肿瘤坏死因子-α 

分 类 号：R733.1[医药卫生—肿瘤]